Mouse IL-18 enzyme-linked immunoassay kit
Catalog# EM0034
Mouse IL-18 enzyme-linked immunoassay kit
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ELISA
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Mouse
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13.72~10000 pg/ml
Overview
Product Name
Mouse IL-18 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
1.46 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0179 | 0.0183 | 0.0181 | |
| 13.72 | 0.0241 | 0.0221 | 0.0231 | 0.0050 |
| 41.15 | 0.0336 | 0.0318 | 0.0327 | 0.0146 |
| 123.46 | 0.0666 | 0.0613 | 0.0640 | 0.0459 |
| 370.37 | 0.1621 | 0.1576 | 0.1599 | 0.1418 |
| 1111.11 | 0.4508 | 0.4650 | 0.4579 | 0.4398 |
| 3333.33 | 1.4010 | 1.3650 | 1.3830 | 1.3649 |
| 10000.00 | 3.6290 | 3.5960 | 3.6125 | 3.5944 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 190.3 | 892.4 | 3058.6 | 188.7 | 879.5 | 3032.9 |
| Standard Deviation | 6.1 | 33.9 | 144.5 | 5.9 | 30.7 | 139.6 |
| Coefficient of Variation (%) | 3.2 | 3.8 | 4.7 | 3.5 | 3.6 | 4.5 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of mouse IL-18 into health mouse serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 108% with an overall mean recovery of 94%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | n.d.-114.63 | 66.7 | 68.74 |
Serum/Plasma – Thirty samples from apparently healthy mice were evaluated for the presence of IL-18 in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Application in Publications
| ELISA | See 7 publications below |
Application in Publications
| Mouse | See 6 publications below |
Target
UNIPROT
Alias
Iboctadekin
IFN gamma inducing factor
IFN-gamma-inducing factor
IGIF
IL 1 gamma
IL 18
IL 1g
IL-1 gamma
IL-18
IL1 gamma
ExpandFunction
Citation
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A high-throughput reporter assay for screening potential gasdermin D inhibitors from natural products
Journal: International Journal Of Biological Macromolecules
DOI: 10.1016/j.ijbiomac.2026.150110
IF: 8.5
Application: ELISA
Reactivity: Mouse
Publish date: 2026 Jan
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METTL3-mediated m6A on nascent RNA coordinates translational and transcriptional programs to activate the NLRP3 inflammasome in macrophages
Journal: Cell Reports
DOI: 10.1016/j.celrep.2025.116808
IF: 6.9
Application: ELISA
Reactivity: Mouse
Publish date: 2026 Jan
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Inhibiting the NLRP3 inflammasome with MCC950 ameliorates muscle atrophy in cancer cachexia
Journal: European Journal Of Pharmacology
DOI: 10.1016/j.ejphar.2026.178654
IF: 4.7
Application: ELISA
Reactivity: Mouse
Publish date: 2026 Feb
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Inhibition of the α7 nicotinic acetylcholine receptor alleviates pyroptosis in blood-brain barrier induced by Cryptococcus neoformans
Journal: Microbial Pathogenesis
DOI: 10.1016/j.micpath.2026.108377
IF: 3.5
Application: ELISA
Reactivity: Mouse
Publish date: 2026 Feb
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Neuromodulation and Copper Chelation Reverse Sleep Fragmentation–Aggravated Myocardial Ischemia-Reperfusion Injury by Targeting NETs-Induced Endothelial Cuproptosis
Journal: Research
DOI: 10.34133/research.1266
IF: 10.7
Application: ELISA
Reactivity:
Publish date: 2026 Apr
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Lipocalin 2 deficiency attenuates NLRP3 inflammasome activation through glycolysis impairment and MGST1-mediated mitochondrial ROS reduction
Journal: International Journal Of Biological Macromolecules
DOI: 10.1016/j.ijbiomac.2025.149021
IF: 8.5
Application: ELISA
Reactivity: Mouse
Publish date: 2025 Nov
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Bacterial infection in weaned piglets promotes diarrhea by inducing the NLRP3 inflammasome-pyroptosis pathway
Journal: Science China-Life Sciences
DOI: 10.1007/s11427-024-2728-2
IF: 9.5
Application: ELISA
Reactivity: Mouse
Publish date: 2025 Jul