Mouse CD163 enzyme-linked immunoassay kit
Catalog# EM0087
Mouse CD163 enzyme-linked immunoassay kit
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ELISA
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Human
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156.25 ~10000 pg/ml
Overview
Product Name
Mouse CD163 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
15.62pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0521 | 0.0460 | 0.0491 | |
| 156.25 | 0.1215 | 0.1155 | 0.1185 | 0.0695 |
| 312.50 | 0.2089 | 0.1872 | 0.1981 | 0.1490 |
| 625.00 | 0.2914 | 0.3162 | 0.3038 | 0.2548 |
| 1250.00 | 0.5026 | 0.5287 | 0.5157 | 0.4666 |
| 2500.00 | 0.8955 | 1.0390 | 0.9673 | 0.9182 |
| 5000.00 | 1.5630 | 1.6870 | 1.6250 | 1.5760 |
| 10000.00 | 2.6230 | 2.8050 | 2.7140 | 2.6650 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| pg/ml | O.D. | Average | Corrected | 0.00 | 0.0521 | |
| Average (pg/ml) | 0.0460 | 0.0491 | 156.25 | 0.1215 | 0.1155 | 0.1185 |
| Standard Deviation | 0.0695 | 312.50 | 0.2089 | 0.1872 | 0.1981 | 0.1490 |
| Coefficient of Variation (%) | 625.00 | 0.2914 | 0.3162 | 0.3038 | 0.2548 | 1250.00 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of mouse CD163 into health mouse serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 95% to 120% with an overall mean recovery of 109%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 185.36-256.77 | 100.0 | 229.54 |
Serum/Plasma– Thirty samples from apparently healthy mice were evaluated for the presence of mouse CD163 in this assay. No medical histories were available for the donors.n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃