Human TNF RI/TNFRSF1A enzyme-linked immunoassay kit（one step）
Catalog# EHY0128
Human TNF RI/TNFRSF1A enzyme-linked immunoassay kit（one step）
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ELISA
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Human
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0.07~50 ng/ml
Overview
Product Name
Human TNF RI/TNFRSF1A enzyme-linked immunoassay kit（one step）
Product Type
Elisa Kit
Sensitivity
0.01 ng/ml (50 μl)；0.13 ng/ml (10 μl)
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0187 | 0.0199 | 0.0193 | |
| 0.07 | 0.0329 | 0.0316 | 0.0323 | 0.0130 |
| 0.21 | 0.0593 | 0.0605 | 0.0599 | 0.0406 |
| 0.62 | 0.1474 | 0.1490 | 0.1482 | 0.1289 |
| 1.85 | 0.3976 | 0.4073 | 0.4025 | 0.3832 |
| 5.56 | 1.0320 | 1.0090 | 1.0205 | 1.0012 |
| 16.67 | 2.1240 | 2.1440 | 2.1340 | 2.1147 |
| 50.00 | 3.4340 | 3.3810 | 3.4075 | 3.3882 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (ng/ml) | 1.1 | 5.9 | 17.9 | 1.1 | 5.4 | 16.8 |
| Standard Deviation | 0.0 | 0.3 | 1.3 | 0.0 | 0.2 | 0.5 |
| Coefficient of Variation (%) | 4.1 | 5.7 | 7.0 | 3.9 | 3.1 | 2.9 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human TNF RI/TNFRSF1A into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 96% to 128% with an overall mean recovery of 120%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 2.4-5.14 | 100 | 3.87 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of TNF RI/TNFRSF1A in this assay. No medical histories were available for the donors.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃