Human EphA2 enzyme-linked immunoassay kit

Usd: 499 Special Discount

Specification

96T

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Catalog# EH0193

Human EphA2 enzyme-linked immunoassay kit

Application

ELISA

Reactivity

Human

Standard curve range

2.74~2000 pg/ml

Overview

Product Name

Human EphA2 enzyme-linked immunoassay kit

Product Type

Elisa Kit

Sensitivity

0.30 pg/ml (50 μl)；1.23 pg/ml (10 μl)；

pg/ml	O.D.		Average	Corrected
0.00	0.0096	0.0093	0.0095
2.74	0.0181	0.0170	0.0176	0.0081
8.23	0.0356	0.0318	0.0337	0.0243
24.69	0.0857	0.0837	0.0847	0.0753
74.07	0.2362	0.2246	0.2304	0.2210
222.22	0.6780	0.6623	0.6702	0.6607
666.67	1.6000	1.5570	1.5785	1.5691
2000.00	3.0380	3.0070	3.0225	3.0131

Precision

	Intra-assay Precision			Inter-assay Precision
Sample Number	S1	S2	S3	S1	S2	S3
Sample Number	22	22	22	6	6	6
Average (pg/ml)	48.5	221.8	684.9	40.3	204.8	657.6
Standard Deviation	3.2	16.2	29.7	2.9	5.7	27.7
Coefficient of Variation (%)	6.5	7.3	4.3	7.2	2.8	4.2

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.

Spike Recovery

The spike recovery was evaluated by spiking 3 levels of human EphA2 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 92% to 102% with an overall mean recovery of 96%.

Sample Value

Sample Matrix	Sample Evaluated	Range (pg/ml)	Detectable (%)	Mean of Detectable (pg/ml)
Serum	30	63.79-146.37	100	91.12

Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of EphA2 in this assay. No medical histories were available for the donors.

Experiment Type

Sandwich Elisa

Species Reactivity

Human

Detection Method

Colorimetric

Operation Duration

120min

Standard Curve Gradient

7 Points/3 Folds

Incubation Count

Detectable Sample

Dosing Volume

50 μl/10 μl

Product Features

Storage Condition

Store at 2-8 ℃

Target

UNIPROT

SwissProt: P29317 Human

Alias

ARCC2

AW545284

CTPA

CTPP1

CTRCT6

EC 2.7.10.1

Eck

Eph receptor A2

EPHA2

EPHA2_HUMAN

Expand

Function

EphA2, also known as Eck, Myk2, and Sek2, is a member of the Eph receptor tyrosine kinase family which binds Ephrins A1, 2, 3, 4, and 5. A and B class Eph proteins have a common structural organization. The human EphA2 cDNA encodes a 976 amino acid (aa) precursor including a 24 aa signal sequence, a 510 aa extracellular domain (ECD), a 24 aa transmembrane segment, and a 418 aa cytoplasmic domain. The ECD contains an N-terminal globular domain, a cysteine-rich domain, and two fibronectin type III domains. The cytoplasmic domain contains a juxtamembrane motif with two tyrosine residues, which are the major autophosphorylation sites, a kinase domain, and a sterile alpha motif (SAM). The ECD of human EphA2 shares 90-94% aa sequence identity with mouse, bovine, and canine EphA2, and approximately 45% aa sequence identity with human EphA1, 3, 4, 5, 7, and 8. EphA2 becomes autophosphorylated following ligand binding and then interacts with SH2 domain-containing PI3-kinase to activate MAPK pathways. Reverse signaling is also propagated through the Ephrin ligand. Transcription of EphA2 is dependent on the expression of E-Cadherin, and can be induced by p53 family transcription factors. EphA2 is upregulated in breast, prostate, and colon cancer vascular endothelium. Its ligand, EphrinA1, is expressed by the local tumor cells. In some cases, EphA2 and EphrinA1 are expressed on the same blood vessels. EphA2 signaling cooperates with VEGF receptor signaling in promoting endothelial cell migration . The gene encoding human EphA2 maps to a region on chromosome 1 which is frequently deleted in neuroectodermal tumors.

Human EphA2 enzyme-linked immunoassay kit

Usd: 499 Special Discount

Specification

Overview

Product Name

Product Type

Sensitivity

Precision

Spike Recovery

Sample Value

Experiment Type

Species Reactivity

Detection Method

Operation Duration

Standard Curve Gradient

Incubation Count

Detectable Sample

Dosing Volume

Product Features

Storage Condition

Target

UNIPROT

Alias

Function

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