Human Angiogenin enzyme-linked immunoassay kit(one step)
Catalog# EHY0190
Human Angiogenin enzyme-linked immunoassay kit(one step)
Application
-
ELISA
Reactivity
-
Human
Standard curve range
-
0.41~100 ng/ml
Overview
Product Name
Human Angiogenin enzyme-linked immunoassay kit(one step)
Product Type
Elisa Kit
Sensitivity
0.01 ng/ml (50 μl)；0.07 ng/ml (10 μl)；
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0123 | 0.0123 | 0.0123 | |
| 0.41 | 0.0423 | 0.0436 | 0.0430 | 0.0307 |
| 1.02 | 0.0915 | 0.0921 | 0.0918 | 0.0795 |
| 2.56 | 0.2093 | 0.2128 | 0.2111 | 0.1988 |
| 6.40 | 0.5093 | 0.5195 | 0.5144 | 0.5021 |
| 16.00 | 1.2320 | 1.2210 | 1.2265 | 1.2142 |
| 40.00 | 2.5030 | 2.4640 | 2.4835 | 2.4712 |
| 100.00 | 3.4960 | 3.4790 | 3.4875 | 3.4752 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (ng/ml) | 2.2 | 9.9 | 34.2 | 2.4 | 10.6 | 35.4 |
| Standard Deviation | 0.1 | 0.1 | 0.6 | 0.0 | 0.2 | 0.6 |
| Coefficient of Variation (%) | 2.3 | 1.4 | 1.6 | 1.1 | 1.7 | 1.7 |
板内精密度：3 个已知浓度的样本酶标板内重复测定 20 次，评估酶标板内的精密度。
板间精密度：3 个已知浓度的样本酶标板间重复检测 6 次，评估酶标板间的精密度。
Spike Recovery
健康人血清加入 3 个不同浓度水平的人 Angiogenin，未加人 Angiogenin 的血清作为本底，计算回收率。回收率的范围从 80 % 至 119 %，平均回收率为 96 %。
Sample Value
| Sample Matrix | Evaluated Sample | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 115.46-277.38 | 100 | 168.44 |
应用本试剂盒，检测 30 个健康志愿者的血清样本，志愿者的用药史不详。n.d. = 测不到浓度值。样本的浓度值低于灵敏度被认为测不到浓度值。
Experiment Type
Sandwich Elisa
Species Reactivity
Human
Detection Method
Colorimetric
Operation Duration
60min
Standard Curve Gradient
7点
Incubation Count
2
Detectable Sample
Dosing Volume
50 μl/10 μl
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
ALS9
AMYOTROPHIC LATERAL SCLEROSIS
ANG 1
ANG
ANG I
ANG1
ANGI
ANGI_HUMAN
Angiogenin
Angiogenin ribonuclease RNase A family, 5
ExpandFunction
血管生成素（ANG）是一种分泌蛋白，属于胰腺核糖核酸酶家族。它最初是基于其在人腺癌细胞系 HT-29 生长条件下的无血清培养基中启动鸡胚绒毛尿囊膜血管化的能力而纯化的。许多其他肿瘤以及正常细胞系也分泌血管生成素。血管生成素存在于正常人血浆中。