Human FABP1/L-FABP enzyme-linked immunoassay kit
Catalog# EH0257
Human FABP1/L-FABP enzyme-linked immunoassay kit
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ELISA
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Human
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312.50~20000 pg/ml
Overview
Product Name
Human FABP1/L-FABP enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
44.07 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0081 | 0.0077 | 0.0079 | |
| 312.50 | 0.0200 | 0.0204 | 0.0202 | 0.0123 |
| 625.00 | 0.0461 | 0.0453 | 0.0457 | 0.0378 |
| 1250.00 | 0.1192 | 0.1184 | 0.1188 | 0.1109 |
| 2500.00 | 0.3383 | 0.3448 | 0.3416 | 0.3337 |
| 5000.00 | 0.8878 | 0.9526 | 0.9202 | 0.9123 |
| 10000.00 | 1.9040 | 1.9400 | 1.9220 | 1.9141 |
| 20000.00 | 2.9330 | 3.0100 | 2.9715 | 2.9636 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 339.4 | 1791.2 | 5777.7 | 422.3 | 1927.0 | 6155.4 |
| Standard Deviation | 16.5 | 78.8 | 264.6 | 21.2 | 52.5 | 175.3 |
| Coefficient of Variation (%) | 4.9 | 4.4 | 4.6 | 5.0 | 2.7 | 2.8 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human FABP1/L-FABP into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 81% to 120% with an overall mean recovery of 95%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| serum | 30 | 3224.34-17741.95 | 100.0 | 6460.29 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of human FABP1/L-FABP in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃