Human CD328/Siglec-7 enzyme-linked immunoassay kit

Usd: 499 Special Discount

Specification

96T

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Catalog# EH0163

Human CD328/Siglec-7 enzyme-linked immunoassay kit

Application

ELISA

Reactivity

Human

Standard curve range

6.86~5000 pg/ml

Overview

Product Name

Human CD328/Siglec-7 enzyme-linked immunoassay kit

Product Type

Elisa Kit

Sensitivity

0.044 pg/ml (50 μl)；2.95 pg/ml (10 μl)；

pg/ml	O.D.		Average	Corrected
0.00	0.0088	0.0084	0.0086
6.86	0.0141	0.0135	0.0138	0.0052
20.58	0.0260	0.0259	0.0260	0.0174
61.73	0.0678	0.0706	0.0692	0.0606
185.19	0.1837	0.1752	0.1795	0.1709
555.56	0.5584	0.5248	0.5416	0.5330
1666.67	1.8760	1.8020	1.8390	1.8304
5000.00	3.9220	3.9817	3.9519	3.9433

Precision

	Intra-assay Precision			Inter-assay Precision
Sample Number	S1	S2	S3	S1	S2	S3
Sample Number	22	22	22	6	6	6
Average (pg/ml)	271.8	787.6	2000.3	108.3	524.4	1723.3
Standard Deviation	7.1	32.9	74.4	6.0	22.1	64.1
Coefficient of Variation (%)	2.6	4.2	3.7	5.5	4.2	3.7

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.

Spike Recovery

The spike recovery was evaluated by spiking 3 levels of human CD328/Siglec-7 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 95% to 114% with an overall mean recovery of 105%.

Sample Value

Sample Matrix	Sample Evaluated	Range (pg/ml)	Detectable (%)	Mean of Detectable (pg/ml)
Serum	30	1491.12-2812.24	100	1982.8

Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of CD328/Siglec-7 in this assay. No medical histories were available for the donors.

Experiment Type

Sandwich Elisa

Species Reactivity

Human

Detection Method

Colorimetric

Operation Duration

120min

Standard Curve Gradient

7 Points/3 Folds

Incubation Count

Detectable Sample

Dosing Volume

50 μl/10 μl

Product Features

Storage Condition

Store at 2-8 ℃

Target

UNIPROT

SwissProt: Q9Y286 Human

Alias

Adhesion inhibitory receptor molecule 1

siglec 7

AIRM 1

AIRM-1

AIRM1

CD328

CD328 antigen

CDw328

D siglec

Expand

Function

Siglecs (sialic acid binding Ig-like lectins) are I-type (Ig-type) lectins belonging to the Ig superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by varying numbers of Ig-like C2-type domains. Eleven human Siglecs have been cloned and characterized. They are sialoadhesin/CD169/Siglec-1, CD22/Siglec-2, CD33/Siglec-3, Myelin-Associated Glycoprotein (MAG/Siglec-4a) and Siglecs 5 to 11. To date, no Siglec has been shown to recognized any cell surface ligand other than sialic acids, suggesting that interactions with glycans containing this carbohydrate are important in mediating the biological functions of Siglecs. Siglecs 5 to 11 share a high degree of sequence similarity with CD33/Siglec-3 both in their extracellular and intracellular regions. They are collectively referred to as CD33-related Siglecs. One remarkable feature of the CD33-related Siglecs is their differential expression pattern within the hematopoietic system. This fact, together with the presence of two conserved immunoreceptor tyrosine-based inhibition motifs (ITIMs) in their cytoplasmic tails, suggests that CD33-related Siglecs are involved in the regulation of cellular activation within the immune system. Human Siglec-7 encodes a 467 amino acid (aa) polypeptide with a hydrophobic signal peptide, an N-terminal Ig-like V-type domain, two Ig-like C2-type domains, a transmembrane region and a cytoplasmic tail. Siglec-7 exists as a monomer on the cell surface and is expressed on natural killer cells, CD8+ T cells and monocytes. It binds equally well to both alpha 2,3- and alpha 2,6-linked sialic acid. The gene encoding Siglec-7 was mapped to chromosome 19q13.3.

Human CD328/Siglec-7 enzyme-linked immunoassay kit

Usd: 499 Special Discount

Specification

Overview

Product Name

Product Type

Sensitivity

Precision

Spike Recovery

Sample Value

Experiment Type

Species Reactivity

Detection Method

Operation Duration

Standard Curve Gradient

Incubation Count

Detectable Sample

Dosing Volume

Product Features

Storage Condition

Target

UNIPROT

Alias

Function

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