Human PDGF-AA enzyme-linked immunoassay kit
Catalog# EH0058
Human PDGF-AA enzyme-linked immunoassay kit
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ELISA
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Human
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0.31~20 ng/ml
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unconjugated
Overview
Product Name
Human PDGF-AA enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.10 ng/ml (50 μl)；0.17 ng/ml (10 μl)
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.2311 | 0.2403 | 0.2357 | |
| 0.31 | 0.2942 | 0.3011 | 0.2977 | 0.0619 |
| 0.63 | 0.3271 | 0.3783 | 0.3527 | 0.1170 |
| 1.25 | 0.4328 | 0.4453 | 0.4391 | 0.2034 |
| 2.50 | 0.6121 | 0.6172 | 0.6147 | 0.3790 |
| 5.00 | 1.0300 | 1.0830 | 1.0565 | 0.8208 |
| 10.00 | 1.8060 | 1.8730 | 1.8395 | 1.6038 |
| 20.00 | 3.0540 | 3.0790 | 3.0665 | 2.8308 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (ng/ml) | 0.41 | 2.16 | 6.48 | 0.50 | 2.35 | 6.85 |
| Standard Deviation | 0.03 | 0.11 | 0.29 | 0.03 | 0.15 | 0.35 |
| Coefficient of Variation (%) | 6.7 | 5.2 | 4.5 | 7.0 | 6.2 | 5.1 |
板内精密度：3 个已知浓度的样本酶标板内重复测定 20 次，评估酶标板内的精密度。
板间精密度：3 个已知浓度的样本酶标板间重复检测 6 次，评估酶标板间的精密度。
Spike Recovery
健康人血清加入 3 个不同浓度水平的人PDGF-AA，未加人 PDGF-AA 的血清作为本底，计算回收率。回收率的范围从 98 % 至 112 %，平均回收率为 106 %。
Sample Value
| Sample Matrix | Evaluated Sample | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 21.10-57.89 | 100 | 33.58 |
应用本试剂盒，检测 30 个健康志愿者的血清样本，志愿者的用药史不详。n.d. = 测不到浓度值。样本的浓度值低于灵敏度被认为测不到浓度值。
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
PDGF A
PDGF A chain
PDGF subunit A
PDGF-1
PDGF1
PDGFA
PDGFA_HUMAN
Platelet derived growth factor alpha chain
Platelet derived growth factor alpha isoform 2 preproprotein
Platelet derived growth factor alpha polypeptide
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