Human TACI/TNFRSF13B enzyme-linked immunoassay kit
Catalog# EH0264
Human TACI/TNFRSF13B enzyme-linked immunoassay kit
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ELISA
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Human
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0.69~500 pg/ml
Overview
Product Name
Human TACI/TNFRSF13B enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.16 pg/ml (50 μl)；1.13 pg/ml (10 μl)；
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0081 | 0.0079 | 0.0080 | |
| 0.69 | 0.0137 | 0.0125 | 0.0131 | 0.0051 |
| 2.06 | 0.0251 | 0.0244 | 0.0248 | 0.0168 |
| 6.17 | 0.0638 | 0.0618 | 0.0628 | 0.0548 |
| 18.52 | 0.1835 | 0.1750 | 0.1793 | 0.1713 |
| 55.56 | 0.5140 | 0.5085 | 0.5113 | 0.5033 |
| 166.67 | 1.4020 | 1.3400 | 1.3710 | 1.3630 |
| 500.00 | 3.0470 | 3.0110 | 3.0290 | 3.0210 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 10.4 | 48.7 | 168.2 | 9.8 | 47.0 | 158.1 |
| Standard Deviation | 0.3 | 1.1 | 8.1 | 0.7 | 1.9 | 10.8 |
| Coefficient of Variation (%) | 2.7 | 2.3 | 4.8 | 6.7 | 4.0 | 6.9 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human TACI/TNFRSF13B into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 90% to 120% with an overall mean recovery of 114%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 79.81-196.15 | 100 | 112.17 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of human TACI/TNFRSF13B in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃