Human CXCL6/GCP-2 enzyme-linked immunoassay kit
Catalog# EH0165
Human CXCL6/GCP-2 enzyme-linked immunoassay kit
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ELISA
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Human
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31.25~2000 pg/ml
Overview
Product Name
Human CXCL6/GCP-2 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
4.34 pg/ml (50 μl)；13.58 pg/ml (10 μl)；
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0073 | 0.0077 | 0.0075 | |
| 31.25 | 0.0185 | 0.0197 | 0.0191 | 0.0116 |
| 62.50 | 0.0450 | 0.0437 | 0.0444 | 0.0369 |
| 125.00 | 0.1488 | 0.1519 | 0.1504 | 0.1429 |
| 250.00 | 0.5995 | 0.6087 | 0.6041 | 0.5966 |
| 500.00 | 1.6640 | 1.7040 | 1.6840 | 1.6765 |
| 1000.00 | 2.9450 | 2.9480 | 2.9465 | 2.9390 |
| 2000.00 | 3.8530 | 3.8660 | 3.8595 | 3.8520 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 139.4 | 425.2 | 854.1 | 107.3 | 323.6 | 710.4 |
| Standard Deviation | 6.7 | 8.8 | 16.6 | 3.6 | 19.4 | 26.5 |
| Coefficient of Variation (%) | 4.8 | 2.1 | 1.9 | 3.4 | 6.0 | 3.7 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human CXCL6/GCP-2 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 90% to 118% with an overall mean recovery of 108%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 18.88-92.95 | 100 | 55.65 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of CXCL6/GCP-2 in this assay. No medical histories were available for the donors.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
C X C motif chemokine 6
Chemokine (C X C motif) ligand 6
Chemokine alpha 3
CKA 3
CKA-3
CKA3
CXCL 6
CXCL6
CXCL6_HUMAN
GCP 2
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