Human Nectin-2 enzyme-linked immunoassay kit
Catalog# EH0208
Human Nectin-2 enzyme-linked immunoassay kit
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ELISA
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Human
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6.86~5000 pg/ml
Overview
Product Name
Human Nectin-2 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.80 pg/ml (50 μl)；3.86 pg/ml (10 μl)；
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0301 | 0.0336 | 0.0319 | |
| 6.86 | 0.0491 | 0.0494 | 0.0493 | 0.0174 |
| 20.58 | 0.0780 | 0.0772 | 0.0776 | 0.0458 |
| 61.73 | 0.1560 | 0.1675 | 0.1618 | 0.1299 |
| 185.19 | 0.4141 | 0.4033 | 0.4087 | 0.3769 |
| 555.56 | 1.0870 | 1.0920 | 1.0895 | 1.0577 |
| 1666.67 | 2.4910 | 2.4590 | 2.4750 | 2.4432 |
| 5000.00 | 4.1673 | 4.2113 | 4.1893 | 4.1575 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 123.6 | 608.0 | 1941.0 | 122.5 | 568.7 | 1788.8 |
| Standard Deviation | 5.3 | 34.8 | 149.9 | 3.3 | 17.4 | 38.6 |
| Coefficient of Variation (%) | 4.3 | 5.7 | 7.7 | 2.7 | 3.1 | 2.2 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human Nectin-2 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 121% with an overall mean recovery of 102%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 2.64-87.89 | 100 | 12.19 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of Nectin-2 in this assay. No medical histories were available for the donors.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
CD 112
CD112
CD112 antigen
Herpes virus entry mediator B
Herpes virus entry protein B
Herpesvirus entry mediator B
Herpesvirus entry protein B
Hve B
HveB
Nectin-2
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