Human TRAF1 enzyme-linked immunoassay kit
Catalog# EH0215
Human TRAF1 enzyme-linked immunoassay kit
Application
-
ELISA
Reactivity
-
Human
Standard curve range
-
20.48~5000 pg/ml
Overview
Product Name
Human TRAF1 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
2.85 pg/ml (50 μl)；7.62 pg/ml (10 μl)；
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0352 | 0.0374 | 0.0363 | |
| 20.48 | 0.0482 | 0.0512 | 0.0497 | 0.0134 |
| 51.20 | 0.0685 | 0.0666 | 0.0676 | 0.0313 |
| 128.00 | 0.1148 | 0.1100 | 0.1124 | 0.0761 |
| 320.00 | 0.2349 | 0.2201 | 0.2275 | 0.1912 |
| 800.00 | 0.5329 | 0.5233 | 0.5281 | 0.4918 |
| 2000.00 | 1.2500 | 1.2230 | 1.2365 | 1.2002 |
| 5000.00 | 2.8350 | 2.7000 | 2.7675 | 2.7312 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 89.7 | 437.9 | 1342.2 | 79.1 | 414.5 | 1371.4 |
| Standard Deviation | 4.9 | 16.7 | 58.5 | 5.9 | 8.8 | 47.9 |
| Coefficient of Variation (%) | 5.5 | 3.8 | 4.4 | 7.4 | 2.1 | 3.5 |
板内精密度：3 个已知浓度的样本酶标板内重复测定 20 次，评估酶标板内的精密度。
板间精密度：3 个已知浓度的样本酶标板间重复检测 6 次，评估酶标板间的精密度。
Spike Recovery
健康人血清加入 3 个不同浓度水平的人 TRAF1 ，未加人 TRAF1 的血清作为本底，计算回收率。回收率的范围从 72 % 至 94 %，平均回收率为 84%。
Sample Value
| Sample Matrix | Evaluated Sample | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | n.d.-43.04 | 63 | 14.45 |
应用本试剂盒，检测 30 个健康志愿者的血清样本，志愿者的用药史不详。n.d. = 测不到浓度值。样本的浓度值低于灵敏度被认为测不到浓度值。
Experiment Type
Sandwich Elisa
Species Reactivity
Human
Detection Method
Colorimetric
Operation Duration
120min
Standard Curve Gradient
7点
Incubation Count
2
Detectable Sample
Dosing Volume
50 μl/10 μl
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
4732496E14Rik
EBI6
EBV induced protein 6
Epstein-Barr virus-induced protein 6
MGC:10353
Tnf receptor associated factor 1
TNF receptor-associated factor 1
TRAF 1
TRAF1
TRAF1_HUMAN
Function
TRAF1（肿瘤坏死因子受体相关因子1）是一种调控炎症与细胞凋亡的细胞内衔接蛋白