Human ICAM-1/CD54 enzyme-linked immunoassay kit（one step）
Catalog# EHY0064
Human ICAM-1/CD54 enzyme-linked immunoassay kit（one step）
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ELISA
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Human
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0.14~100 ng/ml
Overview
Product Name
Human ICAM-1/CD54 enzyme-linked immunoassay kit（one step）
Product Type
Elisa Kit
Sensitivity
1.22 pg/ml (50 μl)；0.05 ng/ml (10 μl)
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0050 | 0.0046 | 0.0048 | |
| 0.14 | 0.0210 | 0.0195 | 0.0203 | 0.0155 |
| 0.41 | 0.0519 | 0.0540 | 0.0530 | 0.0482 |
| 1.23 | 0.1489 | 0.1465 | 0.1477 | 0.1429 |
| 3.70 | 0.4238 | 0.4106 | 0.4172 | 0.4124 |
| 11.11 | 1.0740 | 1.0970 | 1.0855 | 1.0807 |
| 33.33 | 2.3350 | 2.3240 | 2.3295 | 2.3247 |
| 100.00 | 3.1570 | 3.3020 | 3.2295 | 3.2247 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (ng/ml) | 1.8 | 9.0 | 32.7 | 1.7 | 8.7 | 31.0 |
| Standard Deviation | 0.1 | 0.4 | 2.2 | 0.1 | 0.4 | 1.0 |
| Coefficient of Variation (%) | 3.3 | 4.8 | 6.8 | 5.8 | 4.3 | 3.1 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human ICAM-1/CD54 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 83% to 120% with an overall mean recovery of 105%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 55.22-176.34 | 100.0 | 103.75 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of ICAM-1/CD54 in this assay. No medical histories were available for the donors.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
Antigen identified by monoclonal
BB2
BB 2
BB2
CD 54
CD_antigen=CD54
CD54
Cell surface glycoprotein P3.58
Human rhinovirus receptor
ICAM 1
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