Human CXCL1/GRO alpha enzyme-linked immunoassay kit
Catalog# EH0054
Human CXCL1/GRO alpha enzyme-linked immunoassay kit
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ELISA
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Human
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20.48~5000 pg/ml
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unconjugated
Overview
Product Name
Human CXCL1/GRO alpha enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
14.89 pg/ml (50 μl)；32.67 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0137 | 0.0129 | 0.0133 | |
| 20.48 | 0.0170 | 0.0161 | 0.0166 | 0.0033 |
| 51.20 | 0.0261 | 0.0234 | 0.0248 | 0.0115 |
| 128.00 | 0.0446 | 0.0443 | 0.0445 | 0.0312 |
| 320.00 | 0.1492 | 0.1474 | 0.1483 | 0.1350 |
| 800.00 | 0.4857 | 0.5021 | 0.4939 | 0.4806 |
| 2000.00 | 1.2570 | 1.2630 | 1.2600 | 1.2467 |
| 5000.00 | 2.2760 | 2.2510 | 2.2635 | 2.2502 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 119.3 | 509.0 | 1409.9 | 110.0 | 510.1 | 1498.1 |
| Standard Deviation | 9.4 | 39.3 | 109.4 | 5.4 | 27.0 | 100.4 |
| Coefficient of Variation (%) | 7.9 | 7.7 | 7.8 | 4.9 | 5.3 | 6.7 |
板内精密度：3 个已知浓度的样本酶标板内重复测定 20 次，评估酶标板内的精密度。
板间精密度：3 个已知浓度的样本酶标板间重复检测 6 次，评估酶标板间的精密度。
Spike Recovery
健康人血清加入 3 个不同浓度水平的人CXCL1，未加人 CXCL1 的血清作为本底，计算回收率。回收率的范围从 72 % 至 125 %，平均回收率为 101 %。
Sample Value
| Sample Matrix | Evaluated Sample | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 77.28-616.13 | 100 | 358.62 |
应用本试剂盒，检测 30 个健康志愿者的血清样本，志愿者的用药史不详。n.d. = 测不到浓度值。样本的浓度值低于灵敏度被认为测不到浓度值。
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
C-X-C motif chemokine 1
Chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity
alpha)
chemokine (C-X-C motif) ligand 1
CINC-1
CXCL1
Cytokine-induced neutrophil chemoattractant 1
Fibroblast secretory protein
Fsp
Gro 1
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