Human CD27/TNFRSF7 enzyme-linked immunoassay kit
Catalog# EH0114
Human CD27/TNFRSF7 enzyme-linked immunoassay kit
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ELISA
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Human
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13.72~10000 pg/ml
Overview
Product Name
Human CD27/TNFRSF7 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
18.37 pg/ml (50 μl)；3.98 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0211 | 0.0219 | 0.0215 | |
| 13.72 | 0.0346 | 0.0309 | 0.0328 | 0.0113 |
| 41.15 | 0.0708 | 0.0662 | 0.0685 | 0.0470 |
| 123.46 | 0.1491 | 0.1387 | 0.1439 | 0.1224 |
| 370.37 | 0.3997 | 0.3502 | 0.3750 | 0.3535 |
| 1111.11 | 1.0700 | 1.1050 | 1.0875 | 1.0660 |
| 3333.33 | 2.7800 | 2.7570 | 2.7685 | 2.7470 |
| 10000.00 | 4.4793 | 4.4118 | 4.4456 | 4.4241 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 235.3 | 1382.0 | 4675.0 | 207.7 | 1101.3 | 3437.3 |
| Standard Deviation | 16.2 | 90.3 | 227.3 | 10.3 | 68.9 | 259.5 |
| Coefficient of Variation (%) | 6.9 | 6.5 | 4.9 | 5.0 | 6.3 | 7.5 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human CD27/TNFRSF7 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 118% with an overall mean recovery of 103%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 114.75-12084.57 | 100 | 6777.71 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of CD27/TNFRSF7 in this assay. No medical histories were available for the donors.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
CD 27
CD27
CD27 antigen
CD27 molecule
CD27_HUMAN
CD27L receptor
LPFS2
MGC20393
OTTHUMP00000238557
S152
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