Human IL-22 enzyme-linked immunoassay kit
Catalog# EH0086
Human IL-22 enzyme-linked immunoassay kit
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ELISA
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Human
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0.69~500 pg/ml
Overview
Product Name
Human IL-22 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.03 pg/ml (50 μl)；0.21 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0285 | 0.0284 | 0.0285 | |
| 0.69 | 0.0412 | 0.0401 | 0.0407 | 0.0122 |
| 2.06 | 0.0647 | 0.0618 | 0.0633 | 0.0348 |
| 6.17 | 0.1402 | 0.1387 | 0.1395 | 0.1110 |
| 18.52 | 0.3698 | 0.3546 | 0.3622 | 0.3338 |
| 55.56 | 0.9875 | 0.9855 | 0.9865 | 0.9581 |
| 166.67 | 2.4529 | 2.3547 | 2.4038 | 2.3754 |
| 500.00 | 4.1289 | 4.1300 | 4.1295 | 4.1010 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 10.7 | 48.9 | 172.6 | 10.6 | 48.6 | 176.8 |
| Standard Deviation | 0.3 | 1.4 | 5.5 | 0.5 | 2.0 | 8.6 |
| Coefficient of Variation (%) | 2.7 | 2.9 | 3.2 | 4.6 | 4.2 | 4.8 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human IL-22 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 91% to 117% with an overall mean recovery of 101%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 6.48-76 | 100 | 27.25 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of IL-22 in this assay. No medical histories were available for the donors.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
Cytokine Zcyto18
IL 10 related T cell derived inducible factor
IL 21
IL 22
IL D110
IL TIF
IL-10-related T-cell-derived-inducible factor
IL-22
IL-TIF
IL21
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