Human IL-27 enzyme-linked immunoassay kit

Usd: 499 Special Discount

Specification

96T

Bulk Order Quote | Customization Request

Catalog# EH0094

Human IL-27 enzyme-linked immunoassay kit

Application

ELISA

Reactivity

Human

Standard curve range

13.72~10000 pg/ml

Overview

Product Name

Human IL-27 enzyme-linked immunoassay kit

Product Type

Elisa Kit

Sensitivity

5.51 pg/ml (50 μl)；17.28 pg/ml (10 μl)

pg/ml	O.D.		Average	Corrected
0.00	0.0348	0.0396	0.0372
13.72	0.0476	0.0455	0.0466	0.0094
41.15	0.0701	0.0681	0.0691	0.0319
123.46	0.1287	0.1349	0.1318	0.0946
370.37	0.3138	0.3228	0.3183	0.2811
1111.11	0.8036	0.8394	0.8215	0.7843
3333.33	2.1080	2.1650	2.1365	2.0993
10000.00	4.1420	4.2020	4.1720	4.1348

Precision

	Intra-assay Precision			Inter-assay Precision
Sample Number	S1	S2	S3	S1	S2	S3
Sample Number	22	22	22	6	6	6
Average (pg/ml)	205.1	1064.4	3596.5	203.0	1017.0	3373.2
Standard Deviation	5.0	35.7	123.5	11.8	29.4	104.1
Coefficient of Variation (%)	2.4	3.4	3.4	5.8	2.9	3.1

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.

Spike Recovery

The spike recovery was evaluated by spiking 3 levels of human IL-27 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 102% with an overall mean recovery of 91%.

Sample Value

Sample Matrix	Sample Evaluated	Range (pg/ml)	Detectable (%)	Mean of Detectable (pg/ml)
Serum	30	n.d.-405.59	75	68.41

Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of IL-12/IL-23 p40 in this assay. No medical histories were available for the donors.

Experiment Type

Sandwich Elisa

Species Reactivity

Human

Detection Method

Colorimetric

Operation Duration

120min

Standard Curve Gradient

7 Points/3 Folds

Incubation Count

Detectable Sample

Dosing Volume

50 μl/10 μl

Product Features

Storage Condition

Store at 2-8 ℃

Target

UNIPROT

SwissProt: Q8NEV9 Human

Alias

EBI3

IL 27

IL 27 p28 subunit

IL 27A

IL 30

IL-27 subunit alpha

IL-27-A

IL27

IL27-A

IL27A

Expand

Function

IL-27 is a heterodimeric group 2 receptor ligand molecule that belongs to the IL-6/IL-12 family of long type I cytokines. It is composed of EBI3 (EBV-induced gene 3), a 34 kDa glycoprotein that is related to the p40 subunit of IL-12 and IL-23, and p28, the 28 kDa glycoprotein that is related to the p35 chain of IL-12. The human EBI3 gene encodes a 229 amino acid (aa) precursor that contains a 20 aa signal peptide and 209 aa mature protein. The mature region contains two potential N-linked glycosylation sites, two fibronectin type III domains, and two pairs of conserved cysteine residues with a WSXWS-like motif that places the molecule in the hematopoietin receptor family. Although p40, the EBI3 counterpart in IL-12, is known to form homodimers, there is no evidence to date that EBI3 also homodimerizes. Human EBI3 is 61% aa identical to mouse EBI3. The human p28 gene encodes a 243 aa precursor that contains a 28 aa signal sequence and 215 aa mature region. The mature region is characterized by the presence of four alpha -helices, placing it in the IL-6 family of helical cytokines. Human p28 is 74% aa identical to mouse p28. IL-27 is expressed by monocytes, endothelial cells and dendritic cells. IL-27 binds to and signals through a heterodimeric receptor complex composed of WSX-1 (TCCR) and gp130. Evidence suggests IL-27 interacts only with WSX-1. IL-27 has both anti- and proinflammatory properties. As an anti-inflammatory, IL-27 seems to induce a general negative feedback program that limits T and NK-T cell activity. At the onset of infection, IL-27 induces an IL-12 receptor on naïve CD4+ T cells, making them susceptible to subsequent IL-12 activity (and possible Th1 development).

Human IL-27 enzyme-linked immunoassay kit

Usd: 499 Special Discount

Specification

Overview

Product Name

Product Type

Sensitivity

Precision

Spike Recovery

Sample Value

Experiment Type

Species Reactivity

Detection Method

Operation Duration

Standard Curve Gradient

Incubation Count

Detectable Sample

Dosing Volume

Product Features

Storage Condition

Target

UNIPROT

Alias

Function

Same Target and Pathway Products

Human/Mouse MASP2 enzyme-linked immunoassay kit

Human IL-6 Super Sensitivity enzyme-linked immunoassay kit

Human Complement factor B enzyme-linked immunoassay kit(one step)

Human SNAP25 enzyme-linked immunoassay kit

Human CX3CL1/Fractalking enzyme-linked immunoassay kit

Human tPA enzyme-linked immunoassay kit(one step)

Human FGF-19 enzyme-linked immunoassay kit

Human Neuropilin-1/NRP1 enzyme-linked immunoassay kit（one step）

Human/Monkey Complement C3 enzyme-linked immunoassay kit(one step)

Human CD48 enzyme-linked immunoassay kit

Human/Monkey Albumin enzyme-linked immunoassay kit(one step)

Human MMP-7 enzyme-linked immunoassay kit

Human CTLA-4 enzyme-linked immunoassay kit

Human/Monkey/Canine CCL5/RANTES enzyme-linked immunoassay kit(one step)

Human MERTK enzyme-linked immunoassay kit（one step）

Human Lipoprotein A/LPA enzyme-linked immunoassay kit(one step)

Human CTGF/CCN2 enzyme-linked immunoassay kit(one step)

Human Proprotein Convertase 9/PCSK9 enzyme-linked immunoassay kit（one step）

Lentivirus HIV-1 p24 enzyme-linked immunoassay kit（one step）

Human CCL2/MCP-1 enzyme-linked immunoassay kit

Human CCL5/RANTES enzyme-linked immunoassay kit

Human Antithrombin III/AT III enzyme-linked immunoassay kit(one step)

Human CD273/B7-DC/PD-L2 enzyme-linked immunoassay kit（one step）

Human CD73 enzyme-linked immunoassay kit

Human ICAM-1 / CD54 enzyme-linked immunoassay kit

Human CD47 enzyme-linked immunoassay kit

Human IL-17E/IL-25 enzyme-linked immunoassay kit

Human/Monkey/Canine CXCL10/IP-10 enzyme-linked immunoassay kit

Human/Monkey/Canine Osteopontin（OPN） enzyme-linked immunoassay kit

Human Total HSP60 enzyme-linked immunoassay kit

Human IL-13R alpha 2 enzyme-linked immunoassay kit

Human CHIT1 enzyme-linked immunoassay kit

Human RBP4 enzyme-linked immunoassay kit（one step）

Human Caspase-9 enzyme-linked immunoassay kit

Human Granzyme A/GZMA enzyme-linked immunoassay kit

Human IFN-β enzyme-linked immunoassay kit

Human ULBP1/NKG2DL1 enzyme-linked immunoassay kit

Human CD163 enzyme-linked immunoassay kit

Human Renin enzyme-linked immunoassay kit

Human CXCL2/MIP2/GRO beta enzyme-linked immunoassay kit

Human SAA1 enzyme-linked immunoassay kit

Human IL-3 enzyme-linked immunoassay kit

Human/Monkey PCSK9 enzyme-linked immunoassay kit (one step)

Human TGF-α enzyme-linked immunoassay kit

Human Myeloperoxidase(MPO) enzyme-linked immunoassay kit(one step)

Human Leptin enzyme-linked immunoassay kit

Human CD27/TNFRSF7 enzyme-linked immunoassay kit

Human B7-H6/NCR3LG1 enzyme-linked immunoassay kit

Human/Monkey/Canine IL-18 enzyme-linked immunoassay kit

Human Endoglin/CD105 enzyme-linked immunoassay kit

Human IL-1RA/IL-1F3 enzyme-linked immunoassay kit

Human beta-NGF enzyme-linked immunoassay kit

Human IL-15 enzyme-linked immunoassay kit

Human PAI-1/Serpin E1 enzyme-linked immunoassay kit(one step)

Human CD28 enzyme-linked immunoassay kit