Human CCL5/RANTES enzyme-linked immunoassay kit

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Catalog# EH0027

Human CCL5/RANTES enzyme-linked immunoassay kit

Application

ELISA

Reactivity

Human

Standard curve range

2.74~2000 pg/ml

Overview

Product Name

Human CCL5/RANTES enzyme-linked immunoassay kit

Product Type

Elisa Kit

Sensitivity

0.7 pg/ml (50 μl)；4.65 pg/ml (10 μl)

pg/ml	O.D.		Average	Corrected
0.00	0.0166	0.0175	0.0171
2.74	0.0250	0.0273	0.0262	0.0091
8.23	0.0411	0.0383	0.0397	0.0227
24.69	0.0890	0.0927	0.0909	0.0738
74.07	0.2920	0.2875	0.2898	0.2727
222.22	1.0660	1.1230	1.0945	1.0775
666.67	3.1910	3.3870	3.2890	3.2720
2000.00	4.2807	4.3940	4.3374	4.3203

Precision

	Intra-assay Precision			Inter-assay Precision
Sample Number	S1	S2	S3	S1	S2	S3
Sample Number	22	22	22	6	6	6
Average (ng/ml)	0.3	1.5	6.6	0.3	1.5	6.7
Standard Deviation	0.0	0.1	0.3	0.0	0.1	0.4
Coefficient of Variation (%)	5.0	3.4	4.9	5.1	5.6	5.4

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.

Spike Recovery

The spike recovery was evaluated by spiking 3 levels of human CCL5 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 73% to 124% with an overall mean recovery of 97%.

Sample Value

Sample Matrix	Sample Evaluated	Range (ng/ml)	Detectable (%)	Mean of Detectable (ng/ml)
Serum	30	5.88-31.87	100	19.53

Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of CCL5 in this assay. No medical histories were available for the donors.

Experiment Type

Sandwich Elisa

Species Reactivity

Human

Detection Method

Colorimetric

Operation Duration

120min

Standard Curve Gradient

7 Points/3 Folds

Incubation Count

Detectable Sample

Dosing Volume

50 μl/10 μl

Product Features

Storage Condition

Store at 2-8 ℃

Target

UNIPROT

SwissProt: P13501 Human

Alias

Beta chemokine RANTES

Beta chemokine RANTES precursor

C C motif chemokine 5

CCL 5

CCL5

CCL5_HUMAN

Chemokine (C C motif) ligand 5

Chemokine CC Motif Ligand 5

D17S136E

EoCP

Expand

Function

RANTES (Regulated upon Activation, Normal T cell Expressed and presumably Secreted), also known as CCL5, is a member of the "CC" subfamily of chemokines. It plays a primary role in the inflammatory immune response via its ability to chemoattract leukocytes and modulate their function. The cDNA for RANTES was initially discovered by subtractive hybridization as a T cell specific sequence. Human RANTES cDNA encodes a highly basic 91 amino acid (aa) residue precursor polypeptide with a 23 aa hydrophobic signal peptide that is cleaved to generate the 68 aa mature protein. Human RANTES exhibits approximately 85% homology with mouse RANTES at the deduced aa level. RANTES is a potent chemoattractant for a number of different cell types including unstimulated CD4+/CD45RO+ memory T cells and stimulated CD4+ and CD8+ T cells with naive and memory phenotypes, NK cells, basophils, eosinophils, dendritic cells, mast cells, monocytes, and microglia. In addition to its effects on migration, RANTES can activate a number of cell types including T cells, monocytes, neutrophils, NK cells, dendritic cells, and astrocytes. T cell activation generally requires relatively high RANTES concentrations (~ 1 μM) and is dependent upon aggregation of the molecule and association with cell surface glycosaminoglycans (GAGs). Whether this activity occurs in vivo remains unclear although in mice, intraperitoneally injected RANTES mutants that are unable to aggregate and/or bind GAG, are not capable of attracting leukocytes when compared to wild-type controls. Other in vivo studies show that RANTES knockout mice exhibit deficient recruitment of leukocytes to sites of acute inflammation. RANTES, is known to interact with four identified seven transmembrane G-protein coupled receptors: CCR1, CCR3, CCR4, and CCR5 (22-25). RANTES stimulation can initiate a variety of signaling cascades that are cell context dependent. For instance, in T-cells, RANTES can stimulate elevations of intracellular Ca2+, and activation of focal adhesion kinase (FAK), protein kinase A, PI3-kinase, Rho GTPase, and JAK/STAT signaling pathways. The cytomegalovirus protein US28 exhibits significant homology with CC chemokine receptors and is capable of binding RANTES. Membrane-spanning US28 can, depending on the context, signal in a constitutive manner, bind RANTES and initiate G-protein-mediated signaling cascades, or sequester RANTES and potentially alter inflammatory responses. The RANTES receptor CCR5 is also the primary co-receptor for R5 (M-tropic) variants of HIV-1. It has been demonstrated that RANTES, as well as the other CCR5 ligands, macrophage inflammatory protein (MIP)-1 alpha and MIP-1 beta, can competitively inhibit CCR5/HIV-1 interaction and suppress viral infection in vitro. These effects apparently do not require fully intact signaling from the CCR5 receptor. Consequently, modified forms of RANTES and non-peptide compounds that block the interaction of HIV-1 with CCR5 show promise for future therapies. In contrast, several reports show that RANTES can enhance in vitro replication of X4 (T-tropic) variants of HIV-1 that use CXCR4 as a co-receptor rather than CCR5. This activity usually requires relatively high RANTES concentrations (~μM) and is dependent upon interaction with cell surface GAGs, oligomerization, and activation of tyrosine kinase and MAP kinase signaling cascades.

Human CCL5/RANTES enzyme-linked immunoassay kit

Usd: Special Discount

Overview

Product Name

Product Type

Sensitivity

Precision

Spike Recovery

Sample Value

Experiment Type

Species Reactivity

Detection Method

Operation Duration

Standard Curve Gradient

Incubation Count

Detectable Sample

Dosing Volume

Product Features

Storage Condition

Target

UNIPROT

Alias

Function

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