Human PNS-tau enzyme-linked immunoassay kit
Catalog# EH0299
Human PNS-tau enzyme-linked immunoassay kit
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ELISA
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Human
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625.00 ~40000 pg/ml
Overview
Product Name
Human PNS-tau enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
46.13 pg/ml (50 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0293 | 0.0299 | 0.0296 | |
| 625.00 | 0.0625 | 0.0576 | 0.0601 | 0.0305 |
| 1250.00 | 0.0931 | 0.0940 | 0.0936 | 0.0640 |
| 2500.00 | 0.1638 | 0.1644 | 0.1641 | 0.1345 |
| 5000.00 | 0.3227 | 0.3162 | 0.3195 | 0.2899 |
| 10000.00 | 0.6468 | 0.6073 | 0.6271 | 0.5975 |
| 20000.00 | 1.2710 | 1.3110 | 1.2910 | 1.2614 |
| 40000.00 | 2.6410 | 2.6580 | 2.6495 | 2.6199 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 724.5 | 3415.9 | 10995.1 | 729.0 | 3597.6 | 11675.0 |
| Standard Deviation | 31.8 | 147.3 | 412.1 | 22.1 | 107.5 | 365.5 |
| Coefficient of Variation (%) | 4.4 | 4.3 | 3.7 | 3.0 | 3.0 | 3.1 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human PNS-tau into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 90% to 116% with an overall mean recovery of 102%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | n.d.-206.22 | 13.3 | 179.61 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of human PNS-tau in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃