Human Lipoprotein A/LPA enzyme-linked immunoassay kit
Catalog# EH0171
Human Lipoprotein A/LPA enzyme-linked immunoassay kit
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ELISA
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Human
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13.72~10000 pg/ml
Overview
Product Name
Human Lipoprotein A/LPA enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
1.11 pg/ml (50 μl)；7.76 pg/ml (10 μl)；
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0070 | 0.0063 | 0.0067 | |
| 13.72 | 0.0173 | 0.0213 | 0.0193 | 0.0127 |
| 41.15 | 0.0419 | 0.0407 | 0.0413 | 0.0347 |
| 123.46 | 0.1194 | 0.1158 | 0.1176 | 0.1110 |
| 370.37 | 0.3628 | 0.3434 | 0.3531 | 0.3465 |
| 1111.11 | 1.0670 | 1.0320 | 1.0495 | 1.0429 |
| 3333.33 | 2.5740 | 2.5650 | 2.5695 | 2.5629 |
| 10000.00 | 3.9935 | 4.0450 | 4.0193 | 4.0126 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 375.0 | 1408.8 | 3317.3 | 371.3 | 1405.8 | 3425.6 |
| Standard Deviation | 10.0 | 36.1 | 99.1 | 12.8 | 69.1 | 226.0 |
| Coefficient of Variation (%) | 2.7 | 2.6 | 3.0 | 3.4 | 4.9 | 6.6 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human Lipoprotein A/LPA into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 87% to 118% with an overall mean recovery of 98%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 288.08-11292.84 | 100 | 2363.67 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of Lipoprotein A/LPA in this assay. No medical histories were available for the donors.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
AK38
Antiangiogenic AK38 protein
apo(a)
APOA
apolipoprotein(a)
LP
lp(a)
LPA