Human FABP4/A-FABP enzyme-linked immunoassay kit
Catalog# EH0345
Human FABP4/A-FABP enzyme-linked immunoassay kit
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sELISA
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Human
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1.37 ~1000 pg/ml
Overview
Product Name
Human FABP4/A-FABP enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.17pg/ml (50 μl)；1.05pg/ml (10 μl)；
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0209 | 0.0207 | 0.0208 | |
| 1.37 | 0.0276 | 0.0261 | 0.0269 | 0.0061 |
| 4.12 | 0.0456 | 0.0423 | 0.0440 | 0.0232 |
| 12.35 | 0.1056 | 0.1055 | 0.1056 | 0.0848 |
| 37.04 | 0.2834 | 0.2907 | 0.2871 | 0.2663 |
| 111.11 | 0.7984 | 0.8575 | 0.8280 | 0.8072 |
| 333.33 | 2.1060 | 2.1790 | 2.1425 | 2.1217 |
| 1000.00 | 4.0813 | 4.1426 | 4.1120 | 4.0912 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 16.9 | 96.5 | 309.8 | 17.8 | 102.4 | 323.6 |
| Standard Deviation | 1.0 | 4.7 | 20.7 | 0.7 | 6.0 | 18.8 |
| Coefficient of Variation (%) | 6.1 | 4.8 | 6.7 | 3.9 | 5.9 | 5.8 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human FABP4/A-FABP into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 87% to 120% with an overall mean recovery of 105%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 3.33-17.50 | 100 | 6.61 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of human FABP4/A-FABP in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
Adipocyte lipid-binding protein
Adipocyte-type fatty acid-binding protein
Fatty acid-binding protein 4
ALBP
A-FABP
AFABP
FABP4