Human CD137/4-1BB/TNFRSF9 enzyme-linked immunoassay kit
Catalog# EH0124
Human CD137/4-1BB/TNFRSF9 enzyme-linked immunoassay kit
-
ELISA
-
Human
-
15.63~1000 pg/ml
Overview
Product Name
Human CD137/4-1BB/TNFRSF9 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.47 pg/ml (50 μl)；5.07 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0659 | 0.0698 | 0.0679 | |
| 15.63 | 0.1978 | 0.2148 | 0.2063 | 0.1385 |
| 31.25 | 0.3346 | 0.3512 | 0.3429 | 0.2751 |
| 62.50 | 0.5916 | 0.6533 | 0.6225 | 0.5546 |
| 125.00 | 1.1130 | 1.1470 | 1.1300 | 1.0622 |
| 250.00 | 1.8960 | 1.9670 | 1.9315 | 1.8637 |
| 500.00 | 3.2850 | 3.4530 | 3.3690 | 3.3012 |
| 1000.00 | 4.3356 | 4.3766 | 4.3561 | 4.2883 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 25.6 | 117.0 | 352.9 | 22.8 | 110.2 | 319.1 |
| Standard Deviation | 1.4 | 3.5 | 10.2 | 1.1 | 2.3 | 12.4 |
| Coefficient of Variation (%) | 5.3 | 3.0 | 2.9 | 4.7 | 2.1 | 3.9 |
板内精密度：3 个已知浓度的样本酶标板内重复测定 20 次，评估酶标板内的精密度。
板间精密度：3 个已知浓度的样本酶标板间重复检测 6 次，评估酶标板间的精密度。
Spike Recovery
健康人血清加入 3 个不同浓度水平的人 CD137/4-1BB/TNFRSF9，未加人 CD137/4-1BB/TNFRSF9 的血清作为本底，计算回收率。回收率的范围从 86 % 至 116 %，平均回收率为 103 %。
Sample Value
| Sample Matrix | Evaluated Sample | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 20.14-49.28 | 100 | 32.58 |
应用本试剂盒，检测 30 个健康志愿者的血清样本，志愿者的用药史不详。n.d. = 测不到浓度值。样本的浓度值低于灵敏度被认为测不到浓度值。
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
4 1BB
4 1BB ligand receptor
4-1BB ligand receptor
4-1BB Ligand Receptor T Cell
4-1BB, mouse, homolog of
Antigen 4-1BB Homolog
CD 137
CD137
CD137 antigen
CDw137
Expand