Human SOST/Sclerostin enzyme-linked immunoassay kit

Usd: 619 Special Discount

Specification

96T

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Catalog# EH0330

Human SOST/Sclerostin enzyme-linked immunoassay kit

Application

sELISA

Reactivity

Human

Standard curve range

8.19 ~2000 pg/ml

Overview

Product Name

Human SOST/Sclerostin enzyme-linked immunoassay kit

Product Type

Elisa Kit

Sensitivity

0.01 pg/ml (50 μl)；1.99 pg/ml (10 μl)

pg/ml	O.D.		Average	Corrected
0.00	0.0122	0.0117	0.0120
8.19	0.0403	0.0400	0.0402	0.0282
20.48	0.0871	0.0849	0.0860	0.0741
51.20	0.2017	0.2058	0.2038	0.1918
128.00	0.5116	0.4851	0.4984	0.4864
320.00	1.1817	1.2118	1.1968	1.1848
800.00	2.6924	2.6519	2.6722	2.6602
2000.00	4.2647	4.3375	4.3011	4.2892

Precision

	Intra-assay Precision			Inter-assay Precision
Sample Number	S1	S2	S3	S1	S2	S3
Sample Number	22	22	22	6	6	6
Average (pg/ml)	45.4	206.7	657.6	44.1	207.5	653.8
Standard Deviation	1.0	5.8	16.3	0.9	8.8	21.5
Coefficient of Variation (%)	2.2	2.8	2.5	2.1	4.2	3.3

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.

Spike Recovery

The spike recovery was evaluated by spiking 3 levels of human SOST/Sclerostin into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 81% to 109% with an overall mean recovery of 99%.

Sample Value

Sample Matrix	Sample Evaluated	Range (pg/ml)	Detectable (%)	Mean of Detectable (pg/ml)
Serum	30	26.50-325.57	100	112.33

Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of human SOST/Sclerostin in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.

Experiment Type

Sandwich Elisa

Species Reactivity

Human

Detection Method

Colorimetric

Operation Duration

120min

Standard Curve Gradient

7 Points

Incubation Count

Detectable Sample

Dosing Volume

50 μl/10 μl

Product Features

Storage Condition

Store at 2-8 ℃

Target

UNIPROT

SwissProt: Q9BQB4 Human

Alias

BEER

CDD

Cortical hyperostosis with syndactyly

Sclerosteosis

Sclerostin

Sost

SOST_HUMAN

SOST1

UNQ2976/PRO7455/PRO7476

VBCH

Expand

Function

SOST, also known as sclerostin, is a member of the cerberus/DAN family, a group of secreted glycoproteins characterized by a cysteine-knot motif. Cerberus/DAN family members are putative BMP antagonists, and include Dan, Cerberus, Gremlin, PRDC, and Caronte. While the overall sequence identity between members of the family is low, they have conserved spacing of six cysteine residues. Cerberus and Dan have an additional cysteine residue used for dimerization; however, SOST does not and is secreted as a monomer. SOST was originally identified as an important regulator of bone homeostasis. Positional cloning studies identified that mutations in the SOST gene can cause sclerosteosis and van Buchem disease, bone dysplasia disorders characterized by progressive skeletal overgrowth. Significant levels of SOST expression are detected in bone, cartilage, kidney, and liver. SOST is expressed by osteoclasts in developing bones of mouse embryos, including both intramembranously forming skull bones and endochondrally forming long bones. SOST plays a physiological role as a negative regulator of bone formation by repressing BMP-induced osteogenesis. SOST has been shown to have unique ligand specificity, binding BMP-5, -6, and -7 with high affinity and BMP-2 and -4 with low affinity. This seems to be the first example of a BMP antagonist being localized to osteoclasts, cells derived from the hematopoietic lineage, that function to degrade bone matrix. Human and mouse SOST share 88% amino acid identity.

Human SOST/Sclerostin enzyme-linked immunoassay kit

Usd: 619 Special Discount

Specification

Overview

Product Name

Product Type

Sensitivity

Precision

Spike Recovery

Sample Value

Experiment Type

Species Reactivity

Detection Method

Operation Duration

Standard Curve Gradient

Incubation Count

Detectable Sample

Dosing Volume

Product Features

Storage Condition

Target

UNIPROT

Alias

Function

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