Human Fetuin A/AHSG enzyme-linked immunoassay kit
Catalog# EH0320
Human Fetuin A/AHSG enzyme-linked immunoassay kit
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ELISA
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Human
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78.13 ~5000 pg/ml
Overview
Product Name
Human Fetuin A/AHSG enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
1.12 pg/ml (50 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0227 | 0.0230 | 0.0229 | |
| 78.13 | 0.1096 | 0.1081 | 0.1089 | 0.0860 |
| 156.25 | 0.1940 | 0.1943 | 0.1942 | 0.1713 |
| 312.50 | 0.3731 | 0.3664 | 0.3698 | 0.3469 |
| 625.00 | 0.6939 | 0.6896 | 0.6918 | 0.6689 |
| 1250.00 | 1.2690 | 1.2380 | 1.2535 | 1.2307 |
| 2500.00 | 2.2600 | 2.2010 | 2.2305 | 2.2077 |
| 5000.00 | 3.6910 | 3.5980 | 3.6445 | 3.6217 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 69.2 | 427.4 | 1484.1 | 489.9 | 107.6 | 1531.0 |
| Standard Deviation | 2.7 | 18.9 | 75.3 | 10.5 | 2.5 | 43.4 |
| Coefficient of Variation (%) | 3.9 | 4.4 | 5.1 | 2.2 | 2.3 | 2.8 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human Fetuin A/AHSG into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 120% with an overall mean recovery of 101%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (μg/ml) | Detectable (%) | Mean of Detectable (μg/ml) |
|---|---|---|---|---|
| Serum | 30 | 231.16-784.31 | 100 | 330.05 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of human Fetuin A/AHSG in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃