Human Caspase-9 enzyme-linked immunoassay kit
Catalog# EH0225
Human Caspase-9 enzyme-linked immunoassay kit
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ELISA
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Human
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13.72~10000 pg/ml
Overview
Product Name
Human Caspase-9 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.41 pg/ml (50 μl)；4.11 pg/ml (10 μl)；
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0069 | 0.0065 | 0.0067 | |
| 13.72 | 0.0281 | 0.0273 | 0.0277 | 0.0210 |
| 41.15 | 0.0736 | 0.0769 | 0.0753 | 0.0686 |
| 123.46 | 0.2208 | 0.2160 | 0.2184 | 0.2117 |
| 370.37 | 0.6230 | 0.6025 | 0.6128 | 0.6061 |
| 1111.11 | 1.5260 | 1.4650 | 1.4955 | 1.4888 |
| 3333.33 | 2.8990 | 2.9790 | 2.9390 | 2.9323 |
| 10000.00 | 3.9550 | 4.0994 | 4.0272 | 4.0205 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 154.1 | 1139.3 | 2527.1 | 184.8 | 1179.4 | 3612.7 |
| Standard Deviation | 7.2 | 53.5 | 188.9 | 10.2 | 31.3 | 50.6 |
| Coefficient of Variation (%) | 4.7 | 4.7 | 7.5 | 5.5 | 2.7 | 1.4 |
板内精密度：3 个已知浓度的样本酶标板内重复测定 20 次，评估酶标板内的精密度。
板间精密度：3 个已知浓度的样本酶标板间重复检测 6 次，评估酶标板间的精密度。
Spike Recovery
健康人血清加入 3 个不同浓度水平的人 Caspase-9，未加人 Caspase-9 的血清作为本底，计算回收率。回收率的范围从 89 % 至 111 %，平均回收率为 93 %。
Sample Value
| Sample Matrix | Evaluated Sample | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 359.91-4346.98 | 100 | 1798.80 |
应用本试剂盒，检测 30 个健康志愿者的血清样本，志愿者的用药史不详。n.d. = 测不到浓度值。样本的浓度值低于灵敏度被认为测不到浓度值。
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
Caspase9
APAF-3
APAF3
Apoptosis related cysteine peptidase
Apoptotic protease Mch-6
Apoptotic protease-activating factor 3
CASP-9
CASP9
CASP9_HUMAN
Caspase 9 apoptosis related cysteine peptidase
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