Human Caspase-9 enzyme-linked immunoassay kit
Catalog# EH0225
Human Caspase-9 enzyme-linked immunoassay kit
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ELISA
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Human
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13.72~10000 pg/ml
Overview
Product Name
Human Caspase-9 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.41 pg/ml (50 μl)；4.11 pg/ml (10 μl)；
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0069 | 0.0065 | 0.0067 | |
| 13.72 | 0.0281 | 0.0273 | 0.0277 | 0.0210 |
| 41.15 | 0.0736 | 0.0769 | 0.0753 | 0.0686 |
| 123.46 | 0.2208 | 0.2160 | 0.2184 | 0.2117 |
| 370.37 | 0.6230 | 0.6025 | 0.6128 | 0.6061 |
| 1111.11 | 1.5260 | 1.4650 | 1.4955 | 1.4888 |
| 3333.33 | 2.8990 | 2.9790 | 2.9390 | 2.9323 |
| 10000.00 | 3.9550 | 4.0994 | 4.0272 | 4.0205 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 154.1 | 1139.3 | 2527.1 | 184.8 | 1179.4 | 3612.7 |
| Standard Deviation | 7.2 | 53.5 | 188.9 | 10.2 | 31.3 | 50.6 |
| Coefficient of Variation (%) | 4.7 | 4.7 | 7.5 | 5.5 | 2.7 | 1.4 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human Caspase-9 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 89% to 111% with an overall mean recovery of 93%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 359.91-4346.98 | 100 | 1798.80 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of Caspase-9 in this assay. No medical histories were available for the donors.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
Caspase9
APAF-3
APAF3
Apoptosis related cysteine peptidase
Apoptotic protease Mch-6
Apoptotic protease-activating factor 3
CASP-9
CASP9
CASP9_HUMAN
Caspase 9 apoptosis related cysteine peptidase
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