Human HVEM/TNFRSF14 enzyme-linked immunoassay kit
Catalog# EH0107
Human HVEM/TNFRSF14 enzyme-linked immunoassay kit
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ELISA
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Human
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13.72~10000 pg/ml
Overview
Product Name
Human HVEM/TNFRSF14 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
1.68 pg/ml (50 μl)；1.86 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0193 | 0.0194 | 0.0194 | |
| 13.72 | 0.0363 | 0.0381 | 0.0372 | 0.0179 |
| 41.15 | 0.0732 | 0.0746 | 0.0739 | 0.0546 |
| 123.46 | 0.1891 | 0.1851 | 0.1871 | 0.1678 |
| 370.37 | 0.5121 | 0.5123 | 0.5122 | 0.4929 |
| 1111.11 | 1.3600 | 1.3770 | 1.3685 | 1.3492 |
| 3333.33 | 2.8210 | 2.8440 | 2.8325 | 2.8132 |
| 10000.00 | 4.1600 | 4.0765 | 4.1183 | 4.0989 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 222.9 | 1101.3 | 3269.9 | 214.7 | 1006.4 | 3038.2 |
| Standard Deviation | 11.7 | 44.4 | 201.4 | 8.3 | 50.4 | 164.1 |
| Coefficient of Variation (%) | 5.2 | 4.0 | 3.6 | 3.9 | 5.0 | 5.4 |
板内精密度：3 个已知浓度的样本酶标板内重复测定 20 次，评估酶标板内的精密度。
板间精密度：3 个已知浓度的样本酶标板间重复检测 6 次，评估酶标板间的精密度。
Spike Recovery
健康人血清加入 3 个不同浓度水平的人HVEM/TNFRSF14，未加人 HVEM/TNFRSF14 的血清作为本底，计算回收率。回收率的范围从 82 % 至 117 %，平均回收率为 102 %。
Sample Value
| Sample Matrix | Evaluated Sample | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 6823.75-12125.82 | 100 | 8948.2 |
应用本试剂盒，检测 30 个健康志愿者的血清样本，志愿者的用药史不详。n.d. = 测不到浓度值。样本的浓度值低于灵敏度被认为测不到浓度值。
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
HVEML
ATAR
CD270
CD40 like protein precursor
Herpes virus entry mediator A
Herpesvirus entry mediator A
Herpesvirus entry mediator
Herpesvirus entry mediator ligand
HveA
HVEM
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