Human Neurogranin enzyme-linked immunoassay kit
Catalog# EH0210
Human Neurogranin enzyme-linked immunoassay kit
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ELISA
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Human
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8.19~2000 pg/ml
Overview
Product Name
Human Neurogranin enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.92 pg/ml (50 μl)；11.03 pg/ml (10 μl)；
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0112 | 0.0104 | 0.0108 | |
| 8.19 | 0.0162 | 0.0174 | 0.0168 | 0.0060 |
| 20.48 | 0.0261 | 0.0273 | 0.0267 | 0.0159 |
| 51.20 | 0.0538 | 0.0552 | 0.0545 | 0.0437 |
| 128.00 | 0.1319 | 0.1439 | 0.1379 | 0.1271 |
| 320.00 | 0.3888 | 0.4207 | 0.4048 | 0.3940 |
| 800.00 | 1.3030 | 1.2710 | 1.2870 | 1.2762 |
| 2000.00 | 3.5340 | 3.5620 | 3.5480 | 3.5372 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 31.3 | 163.1 | 551.2 | 35.6 | 175.0 | 570.3 |
| Standard Deviation | 1.8 | 8.7 | 23.9 | 1.5 | 5.2 | 10.0 |
| Coefficient of Variation (%) | 5.8 | 5.3 | 4.3 | 4.3 | 3.0 | 1.7 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human Neurogranin into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 119% with an overall mean recovery of 106%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | n.d.-9.44 | 67 | 2.21 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of Neurogranin in this assay. No medical histories were available for the donors.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
Protein kinase C substrate RC3
Calmodulin binding protein
Hng
NEUG(55-78)
NEUG_HUMAN
Neurogranin (protein kinase C substrate)
Ng
NRGN
Protein kinase C substrate
RC3