Human IL-1R2 enzyme-linked immunoassay kit
Catalog# EH0332
Human IL-1R2 enzyme-linked immunoassay kit
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sELISA
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Human
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156.25 ~10000 pg/ml
Overview
Product Name
Human IL-1R2 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
3.24 pg/ml (50 μl)；7.35pg/ml (10 μl)；
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0135 | 0.0145 | 0.0140 | |
| 156.25 | 0.1175 | 0.1120 | 0.1148 | 0.1008 |
| 312.50 | 0.2280 | 0.2201 | 0.2241 | 0.2101 |
| 625.00 | 0.4698 | 0.4395 | 0.4547 | 0.4407 |
| 1250.00 | 0.8763 | 0.8636 | 0.8700 | 0.8560 |
| 2500.00 | 1.6210 | 1.5660 | 1.5935 | 1.5795 |
| 5000.00 | 2.7980 | 2.6830 | 2.7405 | 2.7265 |
| 10000.00 | 4.0427 | 4.0936 | 4.0682 | 4.0542 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 137.9 | 844.8 | 2612.8 | 156.8 | 932.3 | 2846.7 |
| Standard Deviation | 6.4 | 41.2 | 129.3 | 6.7 | 35.4 | 157.6 |
| Coefficient of Variation (%) | 4.7 | 4.9 | 4.9 | 4.2 | 3.8 | 5.5 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human IL-1R2 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 75% to 102% with an overall mean recovery of 94%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 3172.90-7423.33 | 100 | 5663.90 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of human IL-1R2 in this assay. No medical histories were available for the donors.n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
Interleukin-1 receptor type 2
IL-1R-2
IL-1RT-2
IL-1RT2
CD121 antigen-like family member B
CDw121b
IL-1 type II receptor
Interleukin-1 receptor beta (IL-1R-beta)
Interleukin-1 receptor type II
Interleukin-1 receptor type 2, membrane form (mIL-1R2
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