Human RBP4 enzyme-linked immunoassay kit（one step）
Catalog# EHY0212
Human RBP4 enzyme-linked immunoassay kit（one step）
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ELISA
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Human
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0.14~100 ng/ml
Overview
Product Name
Human RBP4 enzyme-linked immunoassay kit（one step）
Product Type
Elisa Kit
Sensitivity
0.002 ng/ml (50 μl)；0.03 ng/ml (10 μl)；
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0087 | 0.0084 | 0.0086 | |
| 0.14 | 0.0340 | 0.0339 | 0.0340 | 0.0254 |
| 0.41 | 0.0873 | 0.0877 | 0.0875 | 0.0790 |
| 1.23 | 0.2276 | 0.2253 | 0.2265 | 0.2179 |
| 3.70 | 0.6074 | 0.5874 | 0.5974 | 0.5889 |
| 11.11 | 1.4550 | 1.3330 | 1.3940 | 1.3855 |
| 33.33 | 3.1820 | 3.2770 | 3.2295 | 3.2210 |
| 100.00 | 4.0330 | 4.2409 | 4.1370 | 4.1284 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (ng/ml) | 1.2 | 9.1 | 33.5 | 1.3 | 12.6 | 34.1 |
| Standard Deviation | 0.1 | 0.5 | 2.5 | 0.1 | 0.4 | 1.4 |
| Coefficient of Variation (%) | 4.9 | 5.8 | 5.4 | 5.9 | 3.1 | 4.0 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human RBP4 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 88% to 118% with an overall mean recovery of 100%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 278.09-774.24 | 100 | 534.25 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of RBP4 in this assay. No medical histories were available for the donors.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
OTTHUMP00000020114
OTTHUMP00000020115
OTTHUMP00000020116
Plasma retinol binding protein 4
Plasma retinol-binding protein
Plasma retinol-binding protein(1-176)
PRBP
PRO2222
RBP
RBP4
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