Human TREM-1 enzyme-linked immunoassay kit
Catalog# EH0227
Human TREM-1 enzyme-linked immunoassay kit
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ELISA
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Human
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13.72~10000 pg/ml
Overview
Product Name
Human TREM-1 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
2.00 pg/ml (50 μl)；2.61 pg/ml (10 μl)；
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0081 | 0.0065 | 0.0073 | |
| 13.72 | 0.0150 | 0.0136 | 0.0143 | 0.0070 |
| 41.15 | 0.0331 | 0.0318 | 0.0325 | 0.0252 |
| 123.46 | 0.0905 | 0.0925 | 0.0915 | 0.0842 |
| 370.37 | 0.2612 | 0.2627 | 0.2620 | 0.2547 |
| 1111.11 | 0.8480 | 0.8700 | 0.8590 | 0.8517 |
| 3333.33 | 2.7722 | 2.7375 | 2.7549 | 2.7476 |
| 10000.00 | 4.5294 | 4.6098 | 4.5696 | 4.5623 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 191.5 | 997.5 | 2959.8 | 190.9 | 991.8 | 2942.6 |
| Standard Deviation | 6.9 | 41.5 | 167.5 | 7.5 | 23.5 | 150.3 |
| Coefficient of Variation (%) | 3.6 | 4.2 | 5.7 | 3.9 | 2.4 | 5.1 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of Human TREM-1 into health serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 93% to 113% with an overall mean recovery of 102%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 92.32-631.59 | 100 | 249.37 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of TREM-1 in this assay. No medical histories were available for the donors.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
CD354
OTTMUSP00000018206
TREM 1
TREM-1
TREM1
TREM1_HUMAN
Triggering receptor expressed on monocytes 1
Triggering receptor expressed on myeloid cells 1
Triggering receptor TREM 1
Triggering receptor TREM1