Human MIP-5/CCL15 enzyme-linked immunoassay kit
Catalog# EH0268
Human MIP-5/CCL15 enzyme-linked immunoassay kit
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ELISA
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Human
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31.25~2000 pg/ml
Overview
Product Name
Human MIP-5/CCL15 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
13.85 pg/ml (50 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0090 | 0.0090 | 0.0090 | |
| 31.25 | 0.0130 | 0.0140 | 0.0135 | 0.0045 |
| 62.50 | 0.0230 | 0.0210 | 0.0220 | 0.0130 |
| 125.00 | 0.0540 | 0.0490 | 0.0515 | 0.0425 |
| 250.00 | 0.1670 | 0.1450 | 0.1560 | 0.1470 |
| 500.00 | 0.5270 | 0.5010 | 0.5140 | 0.5050 |
| 1000.00 | 1.6370 | 1.5760 | 1.6065 | 1.5975 |
| 2000.00 | 3.2740 | 3.2740 | 3.2740 | 3.2650 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 68.2 | 262.6 | 718.7 | 47.7 | 214.8 | 644.6 |
| Standard Deviation | 5.4 | 15.6 | 40.3 | 3.8 | 10.1 | 32.8 |
| Coefficient of Variation (%) | 7.9 | 5.9 | 5.6 | 7.9 | 4.7 | 5.1 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human MIP-5/CCL15 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 85% to 105% with an overall mean recovery of 97%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 2321.43-15417.02 | 100 | 10905.55 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of human MIP-5/CCL15 in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃