Human IL-12p70 enzyme-linked immunoassay kit
Catalog# EH0021
Human IL-12p70 enzyme-linked immunoassay kit
-
ELISA
-
Human
-
13.72~10000 pg/ml
-
unconjugated
Overview
Product Name
Human IL-12p70 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
3.42 pg/ml (50 μl)；11.63 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0432 | 0.0423 | 0.0428 | |
| 13.72 | 0.0532 | 0.0527 | 0.0530 | 0.0102 |
| 41.15 | 0.0731 | 0.0668 | 0.0700 | 0.0272 |
| 123.46 | 0.1270 | 0.1211 | 0.1241 | 0.0813 |
| 370.37 | 0.2712 | 0.2695 | 0.2704 | 0.2276 |
| 1111.11 | 0.6762 | 0.6148 | 0.6455 | 0.6027 |
| 3333.33 | 1.6095 | 1.5049 | 1.5572 | 1.5144 |
| 10000.00 | 3.2771 | 3.3112 | 3.2942 | 3.2514 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 189.2 | 970.0 | 2776.2 | 207.2 | 1027.9 | 3062.6 |
| Standard Deviation | 9.1 | 63.3 | 179.1 | 11.4 | 51.3 | 150.6 |
| Coefficient of Variation (%) | 4.8 | 6.5 | 6.5 | 5.5 | 5.0 | 4.9 |
板内精密度：3 个已知浓度的样本酶标板内重复测定 20 次，评估酶标板内的精密度。
板间精密度：3 个已知浓度的样本酶标板间重复检测 6 次，评估酶标板间的精密度。
Spike Recovery
健康人血清加入 3 个不同浓度水平的人IL-12 p70，未加人 IL-12 p70 的血清作为本底，计算回收率。回收率的范围从 78 % 至 119 %，平均回收率为 98 %。
Sample Value
| Sample Matrix | Evaluated Sample | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 14.8-62.4 | 17 | 36.5 |
应用本试剂盒，检测 30 个健康志愿者的血清样本，志愿者的用药史不详。n.d. = 测不到浓度值。样本的浓度值低于灵敏度被认为测不到浓度值。
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
Alias
CLMF
CLMF p35
Cytotoxic lymphocyte maturation factor 1 p35
Cytotoxic lymphocyte maturation factor 35 kDa subunit
IL 12 subunit p35
IL 12A
IL-12 subunit p35
IL-12A
IL12A
IL12A_HUMAN
Expand