Human Flt3 Ligand/FLT3LG enzyme-linked immunoassay kit

Usd: 499 Special Discount

Specification

96T

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Catalog# EH0255

Human Flt3 Ligand/FLT3LG enzyme-linked immunoassay kit

Application

ELISA

Reactivity

Human

Standard curve range

1.37~1000 pg/ml

Overview

Product Name

Human Flt3 Ligand/FLT3LG enzyme-linked immunoassay kit

Product Type

Elisa Kit

Sensitivity

0.05 pg/ml (50 μl)；1.08 pg/ml (10 μl)；

pg/ml	O.D.		Average	Corrected
0.00	0.0121	0.0112	0.0117
1.37	0.0186	0.0188	0.0187	0.0071
4.12	0.0339	0.0336	0.0338	0.0221
12.35	0.0776	0.0749	0.0763	0.0646
37.04	0.1919	0.1890	0.1905	0.1788
111.11	0.4592	0.5409	0.5001	0.4884
333.33	1.4100	1.4120	1.4110	1.3994
1000.00	3.1510	3.1270	3.1390	3.1274

Precision

	Intra-assay Precision			Inter-assay Precision
Sample Number	S1	S2	S3	S1	S2	S3
Sample Number	22	22	22	6	6	6
Average (pg/ml)	373.36	110.37	22.99	318.3	88.0	19.0
Standard Deviation	25.47	6.27	1.25	10.6	6.6	1.3
Coefficient of Variation (%)	6.8	5.7	5.4	3.3	2.3	6.8

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.

Spike Recovery

The spike recovery was evaluated by spiking 3 levels of human Flt3 Ligand/FLT3LG into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 90% to 119% with an overall mean recovery of 105%.

Sample Value

Sample Matrix	Sample Evaluated	Range (pg/ml)	Detectable (%)	Mean of Detectable (pg/ml)
Serum	30	53.86-214.88	100	101.93

Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated in this assay. No medical histories were available for the donors.

Experiment Type

Sandwich Elisa

Species Reactivity

Human

Detection Method

Colorimetric

Operation Duration

120min

Standard Curve Gradient

7 Points/3 Folds

Incubation Count

Detectable Sample

Dosing Volume

50 μl/10 μl

Product Features

Storage Condition

Store at 2-8 ℃

Target

UNIPROT

SwissProt: P49771 Human

Function

Flt‑3 Ligand, also known as FLT3L, is an alpha-helical cytokine that promotes the differentiation of multiple hematopoietic cell lineages. Mature human Flt‑3 Ligand consists of a 158 amino acid (aa) extracellular domain (ECD) with a cytokine-like domain and a juxtamembrane tether region, a 21 aa transmembrane segment, and a 30 aa cytoplasmic tail. Within the ECD, human Flt‑3 Ligand shares 71% and 65% aa sequence identity with mouse and rat Flt‑3 Ligand, respectively. The human and mouse Flt‑3 Ligand proteins show cross-species activity. Flt-3 Ligand is also structurally related to M-CSF and SCF. Flt-3 Ligand is widely expressed in various human and mouse tissues. It is expressed as a noncovalently-linked dimer by T cells and bone marrow and thymic fibroblasts. Each 36 kDa chain of the Flt-3 Ligand dimer carries approximately 12 kDa of N- and O-linked carbohydrates. Alternate splicing and proteolytic cleavage of the transmembrane form of the Flt-3 Ligand protein can generate a soluble 30 kDa fragment that includes the cytokine-like domain. Alternate splicing of human Flt‑3 Ligand also generates membrane-associated isoforms that contain either a truncated cytoplasmic tail or an 85 aa substitution following the cytokine-like domain in the ECD of the Flt-3 Ligand protein. Both transmembrane and soluble forms of Flt‑3 Ligand signal through the tyrosine kinase receptor Flt-3/Flk-2. Flt‑3 Ligand induces the expansion of monocytes and immature dendritic cells as well as early B cell lineage differentiation. Additionally, Flt-3 Ligand synergizes with IL-3, GM-CSF, and SCF to promote the mobilization and myeloid differentiation of hematopoietic stem cells. Flt-3 Ligand also cooperates with IL-2, IL-6, IL-7, and IL-15 to induce NK cell development and with IL-3, IL-7, and IL-11 to induce terminal B cell maturation. Animal studies show that Flt‑3 Ligand reduces the severity of experimentally induced allergic inflammation .

Human Flt3 Ligand/FLT3LG enzyme-linked immunoassay kit

Usd: 499 Special Discount

Specification

Overview

Product Name

Product Type

Sensitivity

Precision

Spike Recovery

Sample Value

Experiment Type

Species Reactivity

Detection Method

Operation Duration

Standard Curve Gradient

Incubation Count

Detectable Sample

Dosing Volume

Product Features

Storage Condition

Target

UNIPROT

Function

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