Human PD-L1/B7-H1 enzyme-linked immunoassay kit
Catalog# EH0048
Human PD-L1/B7-H1 enzyme-linked immunoassay kit
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sELISA
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Human
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40.96~10000 pg/ml
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unconjugated
Overview
Product Name
Human PD-L1/B7-H1 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
8.50 pg/ml (50 μl)；26.02 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0240 | 0.0272 | 0.0256 | |
| 40.96 | 0.0357 | 0.0431 | 0.0394 | 0.0138 |
| 102.40 | 0.0584 | 0.0637 | 0.0611 | 0.0355 |
| 256.00 | 0.1099 | 0.1093 | 0.1096 | 0.0840 |
| 640.00 | 0.2389 | 0.2377 | 0.2383 | 0.2127 |
| 1600.00 | 0.6145 | 0.6434 | 0.6290 | 0.6034 |
| 4000.00 | 1.6350 | 1.7290 | 1.6820 | 1.6564 |
| 10000.00 | 3.6290 | 3.8300 | 3.7295 | 3.7039 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 209.2 | 1061.1 | 2965.9 | 236.7 | 1178.3 | 3104.7 |
| Standard Deviation | 13.1 | 60.9 | 181.0 | 15.6 | 50.0 | 169.9 |
| Coefficient of Variation (%) | 6.3 | 5.7 | 6.1 | 6.6 | 4.2 | 5.5 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested Twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human PD-L1 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 96% to 127% with an overall mean recovery of 117%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | n.d.-124.46 | 63 | 36.72 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of PD-L1 in this assay. No medical histories were available for the donors.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃