Human ICAM-3/CD50 enzyme-linked immunoassay kit
Catalog# EH0310
Human ICAM-3/CD50 enzyme-linked immunoassay kit
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ELISA
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Human
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40.96 ~10000 pg/ml
Overview
Product Name
Human ICAM-3/CD50 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
7.09pg/ml (50 μl)；15.79 pg/ml (10 μl)；
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0388 | 0.0398 | 0.0393 | |
| 40.96 | 0.0604 | 0.0594 | 0.0599 | 0.0206 |
| 102.40 | 0.0958 | 0.0936 | 0.0947 | 0.0554 |
| 256.00 | 0.1866 | 0.1878 | 0.1872 | 0.1479 |
| 640.00 | 0.3785 | 0.3978 | 0.3882 | 0.3489 |
| 1600.00 | 0.8924 | 0.8582 | 0.8753 | 0.8360 |
| 4000.00 | 1.9740 | 1.9330 | 1.9535 | 1.9142 |
| 10000.00 | 3.5690 | 3.5980 | 3.5835 | 3.5442 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 305.0 | 1111.8 | 3290.7 | 230.0 | 1081.0 | 3271.7 |
| Standard Deviation | 5.7 | 29.7 | 61.8 | 10.0 | 51.1 | 86.0 |
| Coefficient of Variation (%) | 1.9 | 2.7 | 1.9 | 4.4 | 4.7 | 2.6 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human ICAM-3/CD50 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 98% to 114% with an overall mean recovery of 103%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 93.54-214.96 | 100 | 143.28 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of human ICAM-3/CD50 in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃