Human PLGF enzyme-linked immunoassay kit
Catalog# EH0288
Human PLGF enzyme-linked immunoassay kit
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ELISA
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Human
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4.10~1000 pg/ml
Overview
Product Name
Human PLGF enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.83 pg/ml (50 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0377 | 0.0413 | 0.0395 | |
| 4.10 | 0.0677 | 0.0737 | 0.0707 | 0.0312 |
| 10.24 | 0.1165 | 0.1256 | 0.1211 | 0.0816 |
| 25.60 | 0.2566 | 0.2508 | 0.2537 | 0.2142 |
| 64.00 | 0.5458 | 0.5588 | 0.5523 | 0.5128 |
| 160.00 | 1.1780 | 1.1940 | 1.1860 | 1.1465 |
| 400.00 | 2.4310 | 2.4210 | 2.4260 | 2.3865 |
| 1000.00 | 3.9734 | 3.8360 | 3.9047 | 3.8652 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 17.7 | 97.2 | 294.9 | 17.4 | 92.3 | 284.1 |
| Standard Deviation | 0.8 | 3.7 | 11.0 | 0.8 | 4.2 | 10.2 |
| Coefficient of Variation (%) | 4.5 | 3.8 | 3.7 | 4.7 | 4.6 | 3.6 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human PLGF into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 81% to 109% with an overall mean recovery of 93%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| serum | 30 | 7.82-29.54 | 100.0 | 14.90 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of human PLGF in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃