Human CXADR/CAR enzyme-linked immunoassay kit(one step)

Usd: 619 Special Discount

Specification

96T

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Catalog# EHY0262

Human CXADR/CAR enzyme-linked immunoassay kit(one step)

Application

ELISA

Reactivity

Human

Standard curve range

13.72~10000 pg/ml

Overview

Product Name

Human CXADR/CAR enzyme-linked immunoassay kit(one step)

Product Type

Elisa Kit

Sensitivity

2.64 pg/ml (50 μl)；3.77 pg/ml (10 μl)；

pg/ml	O.D.		Average	Corrected
0.00	0.0061	0.0059	0.0060
13.72	0.0100	0.0096	0.0098	0.0038
41.15	0.0194	0.0191	0.0193	0.0133
123.46	0.0491	0.0510	0.0501	0.0441
370.37	0.1454	0.1401	0.1428	0.1368
1111.11	0.4314	0.4186	0.4250	0.4190
3333.33	1.1710	1.1640	1.1675	1.1615
10000.00	2.9580	2.9080	2.9330	2.9270

Precision

	Intra-assay Precision			Inter-assay Precision
Sample Number	S1	S2	S3	S1	S2	S3
Sample Number	22	22	22	6	6	6
Average (pg/ml)	248.5	1141.3	3266.2	246.9	1170.4	3427.4
Standard Deviation	7.2	26.1	129.8	7.3	47.9	102.1
Coefficient of Variation (%)	2.9	2.3	4.0	2.8	3.0	3.0

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.

Spike Recovery

The spike recovery was evaluated by spiking 3 levels of Human CXADR/CAR into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 101% with an overall mean recovery of 95%.

Sample Value

Sample Matrix	Sample Evaluated	Range (pg/ml)	Detectable (%)	Mean of Detectable (pg/ml)
Serum	30	18.04-219.66	100	82.94

Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated in this assay. No medical histories were available for the donors.

Experiment Type

Sandwich Elisa

Species Reactivity

Human

Detection Method

Colorimetric

Operation Duration

60min

Standard Curve Gradient

7 Points/3 Folds

Incubation Count

Detectable Sample

Dosing Volume

50 μl/10 μl

Product Features

Storage Condition

Store at 2-8 ℃

Target

UNIPROT

SwissProt: P78310 Human

Function

CXADR (coxsackie and adenovirus receptor), also known as CAR, is a 46 kDa type I transmembrane glycoprotein that belongs to the CTX family of the Ig superfamily. CXADR has received attention as a receptor that facilitates gene transfer mediated by most adenoviruses. It is also an adhesion molecule within junctional complexes, notably between epithelial cells lining body cavities and within myocardial intercalated discs. CXADR is essential for normal cardiac development in the mouse. It is expressed throughout brain neuroepithelium during development, but mainly in ependymal cells in the adult. The 365 amino acid (aa) mouse CXADR contains a 19 aa signal sequence, a 218 aa extracellular domain (ECD) with a V-type (D1) and a C2-type (D2) Ig-like domain, a 21 aa transmembrane segment and a 107 aa intracellular domain. D1 is thought to be responsible for homodimer formation in trans within tight junctions. The fiber knob of adenoviruses attaches at a similar site, and evidence suggests that disruption of tight junctions facilitates virus binding. A PDZ binding motif at the C-terminus interacts with several cytoplasmic junctional proteins. The ECD of mouse CXADR shares 97%, 90%, 89%, 89% and 88% aa sequence identity with the corresponding regions of rat, human, bovine, porcine and canine CXADR, respectively. An alternately spliced isoform (CXADR2) that diverges in the C-terminal 15 aa shows the same expression pattern, but may show different subcellular localization. Transcription of other splice variants has been detected, but not their translation. A secreted form identified in serum and pleural fluid can block viral infection.

Human CXADR/CAR enzyme-linked immunoassay kit(one step)

Usd: 619 Special Discount

Specification

Overview

Product Name

Product Type

Sensitivity

Precision

Spike Recovery

Sample Value

Experiment Type

Species Reactivity

Detection Method

Operation Duration

Standard Curve Gradient

Incubation Count

Detectable Sample

Dosing Volume

Product Features

Storage Condition

Target

UNIPROT

Function

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