Human Apolipoprotein A1/APOA1 enzyme-linked immunoassay kit
Catalog# EH0319
Human Apolipoprotein A1/APOA1 enzyme-linked immunoassay kit
-
ELISA
-
Human
-
468.75 ~30000 pg/ml
Overview
Product Name
Human Apolipoprotein A1/APOA1 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
91.61pg/ml (50 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0091 | 0.0093 | 0.0092 | |
| 468.75 | 0.0140 | 0.0140 | 0.0140 | 0.0048 |
| 937.50 | 0.0294 | 0.0286 | 0.0290 | 0.0198 |
| 1875.00 | 0.0781 | 0.0818 | 0.0800 | 0.0708 |
| 3750.00 | 0.2654 | 0.2686 | 0.2670 | 0.2578 |
| 7500.00 | 0.8807 | 0.8709 | 0.8758 | 0.8666 |
| 15000.00 | 2.1960 | 2.2280 | 2.2120 | 2.2028 |
| 30000.00 | 4.4280 | 4.2416 | 4.3348 | 4.3256 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 411.0 | 2699.3 | 8436.9 | 609.6 | 2786.8 | 8924.8 |
| Standard Deviation | 24.2 | 110.0 | 280.3 | 21.2 | 15.1 | 427.6 |
| Coefficient of Variation (%) | 5.9 | 4.1 | 3.3 | 3.5 | 0.5 | 4.8 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human Apolipoprotein A1/APOA1 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 93% to 120% with an overall mean recovery of 107%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (μg/ml) | Detectable (%) | Mean of Detectable (μg/ml) |
|---|---|---|---|---|
| Serum | 30 | 683.85-2777.78 | 100 | 1035.81 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of human Apolipoprotein A1/APOA1 in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃