Human Mesothelin enzyme-linked immunoassay kit(one step)
Catalog# EHY0152
Human Mesothelin enzyme-linked immunoassay kit(one step)
Application
-
ELISA
Reactivity
-
Human
Standard curve range
-
1.37~1000 ng/ml
Overview
Product Name
Human Mesothelin enzyme-linked immunoassay kit(one step)
Product Type
Elisa Kit
Sensitivity
0.16 ng/ml (50 μl)；0.23 ng/ml (10 μl)；
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0128 | 0.0150 | 0.0139 | |
| 1.37 | 0.0231 | 0.0235 | 0.0233 | 0.0094 |
| 4.12 | 0.0455 | 0.0442 | 0.0449 | 0.0310 |
| 12.35 | 0.1127 | 0.1187 | 0.1157 | 0.1018 |
| 37.04 | 0.3142 | 0.3075 | 0.3109 | 0.2970 |
| 111.11 | 0.8870 | 0.9468 | 0.9169 | 0.9030 |
| 333.33 | 2.5550 | 2.4420 | 2.4985 | 2.4846 |
| 1000.00 | 4.3296 | 4.4273 | 4.3785 | 4.3646 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (ng/ml) | 22.2 | 107.5 | 332.8 | 19.3 | 97.4 | 295.0 |
| Standard Deviation | 0.8 | 4.5 | 13.4 | 0.8 | 3.5 | 9.2 |
| Coefficient of Variation (%) | 3.5 | 4.2 | 4.0 | 3.9 | 3.5 | 3.1 |
板内精密度：3 个已知浓度的样本酶标板内重复测定 20 次，评估酶标板内的精密度。
板间精密度：3 个已知浓度的样本酶标板间重复检测 6 次，评估酶标板间的精密度。
Spike Recovery
健康人血清加入 3 个不同浓度水平的人 Mesothelin，未加人 Mesothelin 的血清作为本底，计算回收率。回收率的范围从 85 % 至 103 %，平均回收率为 94%。
Sample Value
| Sample Matrix | Evaluated Sample | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 51.87-224.14 | 100 | 119.25 |
应用本试剂盒，检测 30 个健康志愿者的血清样本，志愿者的用药史不详。n.d. = 测不到浓度值。样本的浓度值低于灵敏度被认为测不到浓度值。
Experiment Type
Sandwich Elisa
Species Reactivity
Human
Detection Method
Colorimetric
Operation Duration
60min
Standard Curve Gradient
7点/3倍
Incubation Count
2
Detectable Sample
Dosing Volume
50 μl/10 μl
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
CAK 1
CAK1
CAK1 antigen
cleaved form
Megakaryocyte potentiating factor
Mesothelin
Mesothelin isoform 1 precursor
MPF
Msln
MSLN_HUMAN
ExpandFunction
间皮素，也称为 CAK1 和 ERC，来源于一种前体，该前体还包括巨核细胞增强因子 （MPF）。前体切割后，间皮素通过 GPI 键保持附着在细胞表面。间皮素通常在胸膜、心包和腹膜的间皮细胞以及发育中和出生后的胰腺中表达。它在间皮瘤和一系列癌症和腺瘤中上调。间皮素促进肿瘤细胞增殖、迁移、不依赖锚定的生长和肿瘤进展。