Human LIF enzyme-linked immunoassay kit
Catalog# EH0096
Human LIF enzyme-linked immunoassay kit
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ELISA
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Human
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0.69~500 pg/ml
Overview
Product Name
Human LIF enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.10 pg/ml (50 μl)；0.36 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0142 | 0.0145 | 0.0143 | |
| 0.69 | 0.0244 | 0.0253 | 0.0248 | 0.0105 |
| 2.06 | 0.0432 | 0.0436 | 0.0434 | 0.0291 |
| 6.17 | 0.0940 | 0.0911 | 0.0926 | 0.0782 |
| 18.52 | 0.2485 | 0.2410 | 0.2448 | 0.2304 |
| 55.56 | 0.7105 | 0.6925 | 0.7015 | 0.6872 |
| 166.67 | 1.7840 | 1.6840 | 1.7340 | 1.7197 |
| 500.00 | 3.3730 | 3.4030 | 3.3880 | 3.3737 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 163.4 | 48.5 | 10.5 | 177.7 | 61.2 | 12.2 |
| Standard Deviation | 9.6 | 2.3 | 0.6 | 5.4 | 2.3 | 0.6 |
| Coefficient of Variation (%) | 5.9 | 4.8 | 6.2 | 3.0 | 3.8 | 4.9 |
板内精密度：3 个已知浓度的样本酶标板内重复测定 20 次，评估酶标板内的精密度。
板间精密度：3 个已知浓度的样本酶标板间重复检测 6 次，评估酶标板间的精密度。
Spike Recovery
健康人血清加入 3 个不同浓度水平的人LIF，未加人 LIF的血清作为本底，计算回收率。回收率的范围从 93 % 至 116 %，平均回收率为 102 %。
Sample Value
| Sample Matrix | Evaluated Sample | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 1.85-16.87 | 100 | 4.94 |
应用本试剂盒，检测 30 个健康志愿者的血清样本，志愿者的用药史不详。n.d. = 测不到浓度值。样本的浓度值低于灵敏度被认为测不到浓度值。
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
CDF
Cholinergic Differentiation Factor
D factor
DIA
Differentiation inducing factor
differentiation inhibitory activity
Differentiation stimulating factor
Differentiation-stimulating factor
Emfilermin
Hepatocyte stimulating factor III
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