Human Resistin enzyme-linked immunoassay kit
Catalog# EH0206
Human Resistin enzyme-linked immunoassay kit
-
ELISA
-
Human
-
13.72~10000 pg/ml
Overview
Product Name
Human Resistin enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.33 pg/ml (50 μl)；0.48 pg/ml (10 μl)；
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0118 | 0.0113 | 0.0116 | |
| 13.72 | 0.0165 | 0.0175 | 0.0170 | 0.0055 |
| 41.15 | 0.0270 | 0.0278 | 0.0274 | 0.0159 |
| 123.46 | 0.0605 | 0.0604 | 0.0605 | 0.0489 |
| 370.37 | 0.1887 | 0.1819 | 0.1853 | 0.1738 |
| 1111.11 | 0.5823 | 0.5926 | 0.5875 | 0.5759 |
| 3333.33 | 1.9340 | 1.8680 | 1.9010 | 1.8895 |
| 10000.00 | 4.2792 | 4.3434 | 4.3113 | 4.2998 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 173.5 | 899.9 | 3107.1 | 179.8 | 932.5 | 3171.0 |
| Standard Deviation | 6.8 | 32.8 | 125.6 | 3.0 | 17.6 | 149.7 |
| Coefficient of Variation (%) | 3.9 | 3.6 | 4.0 | 1.7 | 1.9 | 4.7 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human Resistin into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 90% to 116% with an overall mean recovery of 103%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 0.81-34.93 | 100 | 18.17 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of Resistin in this assay. No medical histories were available for the donors.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
Adipose tissue specific secretory factor
Adipose tissue-specific secretory factor
ADSF
C/EBP epsilon regulated myeloid specific secreted cysteine rich protein
C/EBP epsilon regulated myeloid specific secreted cysteine rich protein precursor 1
C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein
CG5403
Cysteine rich secreted protein A12 alpha like 2
Cysteine rich secreted protein FIZZ3
Cysteine-rich secreted protein A12-alpha-like 2
Expand