Human Resistin enzyme-linked immunoassay kit
Catalog# EH0206
Human Resistin enzyme-linked immunoassay kit
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ELISA
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Human
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13.72~10000 pg/ml
Overview
Product Name
Human Resistin enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.33 pg/ml (50 μl)；0.48 pg/ml (10 μl)；
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0118 | 0.0113 | 0.0116 | |
| 13.72 | 0.0165 | 0.0175 | 0.0170 | 0.0055 |
| 41.15 | 0.0270 | 0.0278 | 0.0274 | 0.0159 |
| 123.46 | 0.0605 | 0.0604 | 0.0605 | 0.0489 |
| 370.37 | 0.1887 | 0.1819 | 0.1853 | 0.1738 |
| 1111.11 | 0.5823 | 0.5926 | 0.5875 | 0.5759 |
| 3333.33 | 1.9340 | 1.8680 | 1.9010 | 1.8895 |
| 10000.00 | 4.2792 | 4.3434 | 4.3113 | 4.2998 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 173.5 | 899.9 | 3107.1 | 179.8 | 932.5 | 3171.0 |
| Standard Deviation | 6.8 | 32.8 | 125.6 | 3.0 | 17.6 | 149.7 |
| Coefficient of Variation (%) | 3.9 | 3.6 | 4.0 | 1.7 | 1.9 | 4.7 |
板内精密度：3 个已知浓度的样本酶标板内重复测定 20 次，评估酶标板内的精密度。
板间精密度：3 个已知浓度的样本酶标板间重复检测 6 次，评估酶标板间的精密度。
Spike Recovery
健康人血清加入 3 个不同浓度水平的人 Resistin，未加人 Resistin 的血清作为本底，计算回收率。回收率的范围从 90 % 至 116 %，平均回收率为 103%。
Sample Value
| Sample Matrix | Evaluated Sample | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 0.81-34.93 | 100 | 18.17 |
应用本试剂盒，检测 30 个健康志愿者的血清样本，志愿者的用药史不详。n.d. = 测不到浓度值。样本的浓度值低于灵敏度被认为测不到浓度值。
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
Adipose tissue specific secretory factor
Adipose tissue-specific secretory factor
ADSF
C/EBP epsilon regulated myeloid specific secreted cysteine rich protein
C/EBP epsilon regulated myeloid specific secreted cysteine rich protein precursor 1
C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein
CG5403
Cysteine rich secreted protein A12 alpha like 2
Cysteine rich secreted protein FIZZ3
Cysteine-rich secreted protein A12-alpha-like 2
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