Human Osteoprotegerin/TNFRSF11B enzyme-linked immunoassay kit
Catalog# EH0070
Human Osteoprotegerin/TNFRSF11B enzyme-linked immunoassay kit
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ELISA
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Human
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6.86~5000 pg/ml
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unconjugated
Overview
Product Name
Human Osteoprotegerin/TNFRSF11B enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.37 pg/ml (50 μl)；2.53 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0149 | 0.0139 | 0.0144 | |
| 6.86 | 0.0332 | 0.0309 | 0.0321 | 0.0171 |
| 20.58 | 0.0686 | 0.0681 | 0.0684 | 0.0534 |
| 61.73 | 0.1782 | 0.1770 | 0.1776 | 0.1626 |
| 185.19 | 0.4947 | 0.4959 | 0.4953 | 0.4803 |
| 555.56 | 1.2700 | 1.2650 | 1.2675 | 1.2525 |
| 1666.67 | 2.6800 | 2.5420 | 2.6110 | 2.5960 |
| 5000.00 | 3.8430 | 3.6970 | 3.7700 | 3.7550 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 194.3 | 749.0 | 1558.3 | 209.4 | 735.1 | 1459.6 |
| Standard Deviation | 12.2 | 35.0 | 105.3 | 9.6 | 24.8 | 29.6 |
| Coefficient of Variation (%) | 6.3 | 4.7 | 6.8 | 4.6 | 3.4 | 2.0 |
板内精密度：3 个已知浓度的样本酶标板内重复测定 20 次，评估酶标板内的精密度。
板间精密度：3 个已知浓度的样本酶标板间重复检测 6 次，评估酶标板间的精密度。
Spike Recovery
健康人血清加入 3 个不同浓度水平的人Osteoprotegerin，未加人 Osteoprotegerin 的血清作为本底，计算回收率。回收率的范围从 65 % 至 98 %，平均回收率为 95 %。
Sample Value
| Sample Matrix | Evaluated Sample | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 341.28- 586.42 | 100 | 461.05 |
应用本试剂盒，检测 30 个健康志愿者的血清样本，志愿者的用药史不详。n.d. = 测不到浓度值。样本的浓度值低于灵敏度被认为测不到浓度值。
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
MGC29565
OCIF
OPG
Osteoclastogenesis inhibitory factor
Osteoprotegerin
PDB5
TNF receptor superfamily member 11b
TNFRSF 11B
TNFRSF11B
TR 1
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