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CCL4 / MIP-1 beta Recombinant Rabbit Monoclonal Antibody [JE33-21]

Usd: 385

Specification

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Catalog# HA721904

CCL4 / MIP-1 beta Recombinant Rabbit Monoclonal Antibody [JE33-21]

Application

  • WB

Reactivity

  • Human

  • Mouse

Conjugation

  • unconjugated

Overview

Product Name

CCL4 / MIP-1 beta Recombinant Rabbit Monoclonal Antibody [JE33-21]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human CCL4 / MIP-1 beta aa 1-50 / 92.

Species Reactivity

Human, Mouse

Validated Applications

WB

Molecular Weight

Predicted band size: 10 kDa

Positive Control

THP-1 treated with 100nM TPA overnight then add 100ng/mL LPS for 7 hours then add 1μg/mL BFA for 3 hours cell lysate, RAW264.7 treated with 100nM TPA overnight then add 100ng/mL LPS for 7 hours then add 1μg/mL BFA for 3 hours cell lysate.

Conjugation

unconjugated

Clone Number

JE33-21

RRID

AB_3096754

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

Target

Function

Chemokine (C-C motif) ligands 4 (also CCL4) previously known as macrophage inflammatory protein (MIP-1β), is a protein which in humans is encoded by the CCL4 gene. CCL4 belongs to a cluster of genes located on 17q11-q21 of the chromosomal region. Identification and localization of the gene on the chromosome 17 was in 1990 although the discovery of MIP-1 was initiated in 1988 with the purification of a protein doublet corresponding to inflammatory activity from supernatant of endotoxin-stimulated murine macrophages. At that time, it was also named as "macrophage inflammatory protein-1" (MIP-1) due to its inflammatory properties. CCL4 is a small cytokine that belongs to the CC chemokine subfamily. CCL4 is being secreted under mitogenic signals and antigens and hereby acts as a chemoattractant for natural killer cells, monocytes and various other immune cells in the site of inflamed or damaged tissue.

Background References

1. Zhang L et al. Mangiferin relieves CCl4-induced liver fibrosis in mice. Sci Rep. 2023 Mar

2. Chang TT et al. CCL4 contributes to aging related angiogenic insufficiency through activating oxidative stress and endothelial inflammation. Angiogenesis. 2024 Aug

Subcellular Location

Secreted.

UNIPROT

SwissProt: P13236 Human

SwissProt: Q8NHW4 Human

SwissProt: P14097 Mouse

Synonyms

MIP 1 beta antibody

Secreted protein G 26 antibody

ACT 2 antibody

ACT-2 antibody

ACT2 antibody

AT744.1 antibody

AT744.2 antibody

C C motif chemokine 4 antibody

C C motif chemokine 4 like antibody

C C motif chemokine ligand 4 like 1 antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />Western blot analysis of CCL4 / MIP-1 beta on different lysates with Rabbit anti-CCL4 / MIP-1 beta antibody (<a href="/products/HA721904" style="font-weight: bold;text-decoration: underline;">HA721904</a>) at 1/1,000 dilution.<br /><br />Lane 1: THP-1 cell lysate<br />Lane 2: THP-1 treated with 100nM TPA overnight then add 100ng/mL LPS for 7 hours then add 1μg/mL BFA for 3 hours cell lysate<br />Lane 3: RAW264.7 cell lysate<br />Lane 4: RAW264.7 treated with 100nM TPA overnight then add 100ng/mL LPS for 7 hours then add 1μg/mL BFA for 3 hours cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 10 kDa<br />Observed band size: 10 kDa<br /><br />Exposure time: 1 minute 10 seconds; ECL: K1802;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721904" style="font-weight: bold;text-decoration: underline;">HA721904</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Cell treatment (CT)

    Western blot analysis of CCL4 / MIP-1 beta on different lysates with Rabbit anti-CCL4 / MIP-1 beta antibody (HA721904) at 1/1,000 dilution.

    Lane 1: THP-1 cell lysate
    Lane 2: THP-1 treated with 100nM TPA overnight then add 100ng/mL LPS for 7 hours then add 1μg/mL BFA for 3 hours cell lysate
    Lane 3: RAW264.7 cell lysate
    Lane 4: RAW264.7 treated with 100nM TPA overnight then add 100ng/mL LPS for 7 hours then add 1μg/mL BFA for 3 hours cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 10 kDa
    Observed band size: 10 kDa

    Exposure time: 1 minute 10 seconds; ECL: K1802;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721904) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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Human CCL4 / MIP-1 beta Recombinant Rabbit Monoclonal Antibody [PSH19-49] - BSA and Azide free (Detector)

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