Biotin Conjugated Human CCL4 / MIP-1 beta Recombinant Rabbit Monoclonal Antibody [PSH19-49] - Detector
Overview
Product Name
Biotin Conjugated Human CCL4 / MIP-1 beta Recombinant Rabbit Monoclonal Antibody [PSH19-49] - Detector
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human CCL4 / MIP-1 beta aa 24-92 (HA211213).
Species Reactivity
Human, Cynomolgus monkey
Validated Applications
ELISA(Det), ELISA
Positive Control
Recombinant Human CCL4 / MIP-1 beta protein (HA211213).
Conjugation
Biotin
Clone Number
PSH19-49
Product Features
Form
Liquid
Concentration
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.05% Proclean 300.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA(Det)
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Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH19-48] to Human CCL4 / MIP-1 beta antibody (Capture) (HA725355) and Recombinant Human CCL4 / MIP-1 beta protein (HA211213) as the standard. The reference range value is 15.6-2,000 pg/mL.
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ELISA
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Use at an assay dependent concentration.
Target
Function
Chemokine (C-C motif) ligands 4 (also CCL4) previously known as macrophage inflammatory protein (MIP-1β), is a protein which in humans is encoded by the CCL4 gene. CCL4 belongs to a cluster of genes located on 17q11-q21 of the chromosomal region. Identification and localization of the gene on the chromosome 17 was in 1990 although the discovery of MIP-1 was initiated in 1988 with the purification of a protein doublet corresponding to inflammatory activity from supernatant of endotoxin-stimulated murine macrophages. At that time, it was also named as "macrophage inflammatory protein-1" (MIP-1) due to its inflammatory properties. CCL4 is a small cytokine that belongs to the CC chemokine subfamily. CCL4 is being secreted under mitogenic signals and antigens and hereby acts as a chemoattractant for natural killer cells, monocytes and various other immune cells in the site of inflamed or damaged tissue.
Background References
1. Zhang L et al. Mangiferin relieves CCl4-induced liver fibrosis in mice. Sci Rep. 2023 Mar
2. Chang TT et al. CCL4 contributes to aging related angiogenic insufficiency through activating oxidative stress and endothelial inflammation. Angiogenesis. 2024 Aug
Subcellular Location
Secreted.
UNIPROT
Synonyms
MIP 1 beta antibody
Secreted protein G 26 antibody
ACT 2 antibody
ACT-2 antibody
ACT2 antibody
AT744.1 antibody
AT744.2 antibody
C C motif chemokine 4 antibody
C C motif chemokine 4 like antibody
C C motif chemokine ligand 4 like 1 antibody
ExpandImages
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![Sandwich ELISA analysis of Human CCL4 / MIP-1 beta matched pair antibodies<br /><br />Capture: <a href="/products/HA725355" style="font-weight: bold;text-decoration: underline;">HA725355</a>, Human CCL4 / MIP-1 beta Rabbit mAb [PSH19-48]<br />Detector: <a href="/products/HA725356" style="font-weight: bold;text-decoration: underline;">HA725356</a>, Human CCL4 / MIP-1 beta Rabbit mAb [PSH19-49]<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (<a href="/products/HA725355" style="font-weight: bold;text-decoration: underline;">HA725355</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CCL4 / MIP-1 beta protein (<a href="/products/HA211213" style="font-weight: bold;text-decoration: underline;">HA211213</a>) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (<a href="/products/HA725356" style="font-weight: bold;text-decoration: underline;">HA725356</a>, Biotin, 0.05 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](https://storage.huabio.cn/huabio/productImg/HA725356B_1.png?v=20260401003248)
Sandwich ELISA analysis of Human CCL4 / MIP-1 beta matched pair antibodies
Capture: HA725355, Human CCL4 / MIP-1 beta Rabbit mAb [PSH19-48]
Detector: HA725356, Human CCL4 / MIP-1 beta Rabbit mAb [PSH19-49]
Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725355) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CCL4 / MIP-1 beta protein (HA211213) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA725356, Biotin, 0.05 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. -
![Interpolated concentrations of native CCL4 in TPA and LPS stimulated or unstimulated THP-1 cell culture supernatant.<br /><br />Capture: <a href="/products/HA725355" style="font-weight: bold;text-decoration: underline;">HA725355</a>, Human CCL4 / MIP-1 beta Rabbit mAb [PSH19-48]<br />Detector: <a href="/products/HA725356" style="font-weight: bold;text-decoration: underline;">HA725356</a>, Human CCL4 / MIP-1 beta Rabbit mAb [PSH19-49]<br /><br />THP-1 cells were stimulated overnight with 100 nM TPA, and then stimulated with 100 ng/ml LPS for 7 hours. The concentrations of human CCL4 measured in duplicate and interpolated from the human CCL4 standard curve and corrected for sample dilution. Undiluted samples are as follows: unstimulated 5% and stimulated 5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean human CCL4 concentration was determined to be 13,170.81 pg/ml in TPA and LPS stimulated THP-1 cell culture supernatant and undetectable in the unstimulated THP-1 control.](https://storage.huabio.cn/huabio/productImg/HA725356B_2.png?v=20260401003248)
Interpolated concentrations of native CCL4 in TPA and LPS stimulated or unstimulated THP-1 cell culture supernatant.
Capture: HA725355, Human CCL4 / MIP-1 beta Rabbit mAb [PSH19-48]
Detector: HA725356, Human CCL4 / MIP-1 beta Rabbit mAb [PSH19-49]
THP-1 cells were stimulated overnight with 100 nM TPA, and then stimulated with 100 ng/ml LPS for 7 hours. The concentrations of human CCL4 measured in duplicate and interpolated from the human CCL4 standard curve and corrected for sample dilution. Undiluted samples are as follows: unstimulated 5% and stimulated 5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean human CCL4 concentration was determined to be 13,170.81 pg/ml in TPA and LPS stimulated THP-1 cell culture supernatant and undetectable in the unstimulated THP-1 control.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
Human CCL4 / MIP-1 beta Recombinant Rabbit Monoclonal Antibody [PSH19-48] - BSA and Azide free (Capture)
Application: ELISA(Cap)
Reactivity: Human,Cynomolgus monkey
Conjugate: unconjugated
Human CCL4 / MIP-1 beta Recombinant Rabbit Monoclonal Antibody [PSH19-49] - BSA and Azide free (Detector)
Application: ELISA(Det)
Reactivity: Human,Cynomolgus monkey
Conjugate: unconjugated
