CCR3 Rabbit Polyclonal Antibody
Catalog# HA500279
CCR3 Rabbit Polyclonal Antibody
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WB
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IF-Cell
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Human
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unconjugated
Overview
Product Name
CCR3 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within human CCR3 aa 1-34 / 355.
Species Reactivity
Human
Validated Applications
WB, IF-Cell
Molecular Weight
41 kDa
Positive Control
K562 cell lysate, Jurkat cell lysate, HepG2 cell lysate, SW480.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:500
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IF-Cell
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1:50-1:200
Target
Function
C-C chemokine receptor type 3 is a protein that in humans is encoded by the CCR3 gene. CCR3 has also recently been designated CD193 (cluster of differentiation 193). The protein encoded by this gene is a receptor for C-C type chemokines. It belongs to family 1 of the G protein-coupled receptors. This receptor binds and responds to a variety of chemokines, including eotaxin (CCL11), eotaxin-3 (CCL26), MCP-3 (CCL7), MCP-4 (CCL13), and RANTES (CCL5). It is highly expressed in eosinophils and basophils, and is also detected in TH1 and TH2 cells, as well as in airway epithelial cells. This receptor may contribute to the accumulation and activation of eosinophils and other inflammatory cells in the allergic airway, and possibly at sites of parasitic infection. It is also known to be an entry co-receptor for HIV-1. This gene and seven other chemokine receptor genes form a chemokine receptor gene cluster on the chromosomal region 3p21. Alternatively spliced transcript variants encoding the same protein have been described.
Background References
1. Ponath P.D. et. al. Molecular cloning and characterization of a human eotaxin receptor expressed selectively on eosinophils. J. Exp. Med. 183:2437-2448(1996).
2. Combadiere C. et. al. Cloning and functional expression of a human eosinophil CC chemokine receptor. J. Biol. Chem. 270:16491-16494(1995).
Subcellular Location
Cell membrane.
UNIPROT
Synonyms
B chemokine receptor antibody
b-chemokine receptor antibody
C C chemokine receptor type 3 antibody
C C CKR 3 antibody
C-C chemokine receptor type 3 antibody
C-C CKR-3 antibody
CC chemokine receptor 3 antibody
CC chemokine receptor type 3 antibody
CC CKR 3 antibody
CC R3 antibody
ExpandImages
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Western blot analysis of CCR3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500279, 1/1,000) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: K562 cell lysate
Lane 2: Jurkat cell lysate
Lane 3: HepG2 cell lysate
Predicted band size: 41 kDa
Observed band size: 60 kDa -
ICC staining of CCR3 in SW480 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500279, 1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
CCR3 Rabbit Polyclonal Antibody
Application: IHC-P,IF-Tissue
Reactivity: Human,Rat
Conjugate: unconjugated
