CD116 Rabbit Polyclonal Antibody
Catalog# HA500268
CD116 Rabbit Polyclonal Antibody
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WB
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IF-Cell
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IHC-P
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FC
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Human
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unconjugated
Overview
Product Name
CD116 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within human CD116 aa 351-400 (Cytoplasmic domain).
Species Reactivity
Human
Validated Applications
WB, IF-Cell, IHC-P, FC
Target Molecular Weight
Predicted band size: 46 kDa
Positive Control
Hela cell lysate, Jurkat cell lysate, 293T cell lysate, HepG2 cell lysate, human skin tissue, SiHa, A431, Hela.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:1,000
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IF-Cell
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1:200-1:400
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IHC-P
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1:1,000
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FC
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1:500-1:1,000
Target
Function
The protein encoded by this gene is the alpha subunit of the heterodimeric receptor for colony stimulating factor 2, a cytokine which controls the production, differentiation, and function of granulocytes and macrophages. The encoded protein is a member of the cytokine family of receptors. This gene is found in the pseudoautosomal region (PAR) of the X and Y chromosomes. Multiple transcript variants encoding different isoforms have been found for this gene, with some of the isoforms being membrane-bound and others being soluble.
Background References
1. Huang R. et. al. miR-532-3p-CSF2RA Axis as a Key Regulator of Vulnerable Atherosclerotic Plaque Formation. Can J Cardiol. 2020 Nov
2. Hetzel M. et. al. Function and Safety of Lentivirus-Mediated Gene Transfer for CSF2RA-Deficiency. Hum Gene Ther Methods. 2017 Dec
Subcellular Location
Cell membrane; Secreted.
UNIPROT
Synonyms
CD_antigen=CD116 antibody
CD116 antibody
CD116 antigen antibody
CDw116 antibody
Colony stimulating factor 2 receptor alpha chain antibody
Colony stimulating factor 2 receptor alpha low affinity antibody
Colony stimulating factor 2 receptor alpha subunit antibody
CSF 2R antibody
CSF2R antibody
CSF2R_HUMAN antibody
ExpandImages
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Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-CD116 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500268, 1/1,000) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunocytochemistry analysis of SiHa cells labeling CD116 with Rabbit anti-CD116 antibody (HA500268) at 1/400 dilution.
Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-CD116 antibody (HA500268) at 1/400 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. -
ICC staining of CD116 in A431 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500268, 1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
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ICC staining of CD116 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500268, 1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
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Flow cytometric analysis of Hela cells labeling CD116.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA500268, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). -
Western blot analysis of CD116 on different lysates with Rabbit anti-CD116 antibody (HA500268) at 1/1,000 dilution.
Lane 1: Jurkat (Human T-lymphoblastic cells) cell lysate
Lane 2: 293T (Human embryonic kidney cell) cell lysate
Exposure time: 3 minutes; ECL: K1802
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA500268, 1/1,000 in primary antibody dilution buffer (K1803), 2 hour at room temperature
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 46 kDa
Observed band size: 65 kDa
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Alternative Products
CD116 Recombinant Rabbit Monoclonal Antibody [JE61-37]
Application: WB,IHC-P,IF-Cell
Reactivity: Human
Conjugate: unconjugated
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