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CD116 Recombinant Rabbit Monoclonal Antibody [JE61-37]

Usd: 385

Specification

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Catalog# HA721253

CD116 Recombinant Rabbit Monoclonal Antibody [JE61-37]

Application

  • WB

  • IHC-P

  • IF-Cell

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

CD116 Recombinant Rabbit Monoclonal Antibody [JE61-37]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human CD116 aa 23-122 / 400.

Species Reactivity

Human

Validated Applications

WB, IHC-P, IF-Cell

Molecular Weight

Predicted band size: 46 kDa

Positive Control

JAR cell lysate, Hela cell lysate, 293T cell lysate, human placenta tissue lysate, human malignant melanoma tissue, human skin tissue, SiHa.

Conjugation

unconjugated

Clone Number

JE61-37

RRID

AB_3072370

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500

  • IHC-P

  • 1:1,000

  • IF-Cell

  • 1:50

Target

Function

The protein encoded by this gene is the alpha subunit of the heterodimeric receptor for colony stimulating factor 2, a cytokine which controls the production, differentiation, and function of granulocytes and macrophages. The encoded protein is a member of the cytokine family of receptors. This gene is found in the pseudoautosomal region (PAR) of the X and Y chromosomes. Multiple transcript variants encoding different isoforms have been found for this gene, with some of the isoforms being membrane-bound and others being soluble.

Background References

1. Huang R. et. al. miR-532-3p-CSF2RA Axis as a Key Regulator of Vulnerable Atherosclerotic Plaque Formation. Can J Cardiol. 2020 Nov

2. Hetzel M. et. al. Function and Safety of Lentivirus-Mediated Gene Transfer for CSF2RA-Deficiency. Hum Gene Ther Methods. 2017 Dec

Subcellular Location

Cell membrane; Secreted.

UNIPROT

SwissProt: P15509 Human

Synonyms

CD_antigen=CD116 antibody

CD116 antibody

CD116 antigen antibody

CDw116 antibody

Colony stimulating factor 2 receptor alpha chain antibody

Colony stimulating factor 2 receptor alpha low affinity antibody

Colony stimulating factor 2 receptor alpha subunit antibody

CSF 2R antibody

CSF2R antibody

CSF2R_HUMAN antibody

Expand

Images

  • Western blot analysis of CD116 on different lysates with Rabbit anti-CD116 antibody (<a href="/products/HA721253" style="font-weight: bold;text-decoration: underline;">HA721253</a>) at 1/500 dilution.<br /><br />Lane 1: JAR cell lysate (10 µg/Lane)<br />Lane 2: Hela cell lysate (10 µg/Lane)<br />Lane 3: 293T cell lysate (10 µg/Lane)<br />Lane 4: Human placenta tissue lysate (20 µg/Lane)<br /><br />Predicted band size: 46 kDa<br />Observed band size: 50 kDa<br /><br />Exposure time: 2 minutes;<br /><br />12% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721253" style="font-weight: bold;text-decoration: underline;">HA721253</a>) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:300,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of CD116 on different lysates with Rabbit anti-CD116 antibody (HA721253) at 1/500 dilution.

    Lane 1: JAR cell lysate (10 µg/Lane)
    Lane 2: Hela cell lysate (10 µg/Lane)
    Lane 3: 293T cell lysate (10 µg/Lane)
    Lane 4: Human placenta tissue lysate (20 µg/Lane)

    Predicted band size: 46 kDa
    Observed band size: 50 kDa

    Exposure time: 2 minutes;

    12% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721253) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue with Rabbit anti-CD116 antibody (<a href="/products/HA721253" style="font-weight: bold;text-decoration: underline;">HA721253</a>) at 1/500 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721253" style="font-weight: bold;text-decoration: underline;">HA721253</a>) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue with Rabbit anti-CD116 antibody (HA721253) at 1/500 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721253) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-CD116 antibody (<a href="/products/HA721253" style="font-weight: bold;text-decoration: underline;">HA721253</a>) at 1/500 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721253" style="font-weight: bold;text-decoration: underline;">HA721253</a>) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-CD116 antibody (HA721253) at 1/500 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721253) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunocytochemistry analysis of SiHa cells labeling CD116 with Rabbit anti-CD116 antibody (<a href="/products/HA721253" style="font-weight: bold;text-decoration: underline;">HA721253</a>) at 1/50 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-CD116 antibody (<a href="/products/HA721253" style="font-weight: bold;text-decoration: underline;">HA721253</a>) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.<br /><br />Beta tubulin (<a href="/products/M1305-2" style="font-weight: bold;text-decoration: underline;">M1305-2</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 594, <a href="/products/HA1126" style="font-weight: bold;text-decoration: underline;">HA1126</a>) was used as the secondary antibody at 1/1,000 dilution.

    Immunocytochemistry analysis of SiHa cells labeling CD116 with Rabbit anti-CD116 antibody (HA721253) at 1/50 dilution.

    Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-CD116 antibody (HA721253) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

    Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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metafield image

CD116 Rabbit Polyclonal Antibody

Application: WB,IF-Cell,IHC-P,FC

Reactivity: Human

Conjugate: unconjugated