CD13 Recombinant Rabbit Monoclonal Antibody [PD00-39]
Catalog# HA721160
CD13 Recombinant Rabbit Monoclonal Antibody [PD00-39]
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IHC-P
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IF-Tissue
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WB
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Human
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Mouse
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unconjugated
Overview
Product Name
CD13 Recombinant Rabbit Monoclonal Antibody [PD00-39]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within N Terminal human CD13.
Species Reactivity
Human, Mouse
Validated Applications
IHC-P, IF-Tissue, WB
Molecular Weight
Predicted band size: 110 kDa
Positive Control
Human tonsil tissue, human spleen tissue, human kidney tissue, mouse kidney tissue, PANC-1 cell lysate, THP-1 cell lysate, U-937 cell lysate, HL-60 cell lysate, Mouse kidney tissue lysate.
Conjugation
unconjugated
Clone Number
PD00-39
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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IHC-P
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1:1,000-1:20,000
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IF-Tissue
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1:1,000
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WB
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1:2,000
Target
Function
Aminopeptidase N is located in the small-intestinal and renal microvillar membrane, and also in other plasma membranes. In the small intestine aminopeptidase N plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. Its function in proximal tubular epithelial cells and other cell types is less clear. The large extracellular carboxyterminal domain contains a pentapeptide consensus sequence characteristic of members of the zinc-binding metalloproteinase superfamily. Sequence comparisons with known enzymes of this class showed that CD13 and aminopeptidase N are identical. The latter enzyme was thought to be involved in the metabolism of regulatory peptides by diverse cell types, including small intestinal and renal tubular epithelial cells, macrophages, granulocytes, and synaptic membranes from the CNS. This membrane-bound zinc metalloprotease is known to serve as a receptor for the HCoV-229E alphacoronavirus as well as other non-human coronaviruses. This gene has also been shown to promote angiogenesis, tumor growth, and metastasis and defects in this gene are associated with various types of leukemia and lymphoma.
Background References
1. Ghosh M. et. al. CD13 is a critical regulator of cell-cell fusion in osteoclastogenesis. Sci Rep. 2021 May
2. Lu C. et. al. CD13/Aminopeptidase N Is a Potential Therapeutic Target for Inflammatory Disorders. J Immunol. 2020 Jan
Subcellular Location
Cell membrane.
Synonyms
Alanyl (membrane) aminopeptidase antibody
Alanyl aminopeptidase antibody
Aminopeptidase M antibody
Aminopeptidase N antibody
AMPN_HUMAN antibody
ANPEP antibody
AP M antibody
AP N antibody
AP-M antibody
AP-N antibody
ExpandImages
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Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-CD13 antibody (HA721160) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721160) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-CD13 antibody (HA721160) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721160) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-CD13 antibody (HA721160) at 1/20,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721160) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-CD13 antibody (HA721160) at 1/20,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721160) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Application: IF-Tissue
Species: Mouse
Site: kidney
Sample: Paraffin-embedded section
Antibody concentration: 1/1,000 -
☑ Relative expression (RE)
Western blot analysis of CD13 on different lysates with Rabbit anti-CD13 antibody (HA721160) at 1/2,000 dilution.
Lane 1: PANC-1 cell lysate (5 µg/Lane)
Lane 2: THP-1 cell lysate (30 µg/Lane)
Lane 3: U-937 cell lysate (20 µg/Lane)
Lane 4: 293T cell lysate (negative) (20 µg/Lane)
Lane 5: HL-60 cell lysate (20 µg/Lane)
Lane 6: Mouse kidney tissue lysate (20 µg/Lane)
Predicted band size: 110 kDa
Observed band size: 150 kDa
Exposure time: Lane 1: 9 seconds; Lane 2-5: 24 seconds; Lane 6: 1 minute 45 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721160) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Knockdown (KD)
Western blot analysis of CD13 on different lysates with Rabbit anti-CD13 antibody (HA721160) at 1/2,000 dilution.
Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-CD13 KD cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 110 kDa
Observed band size: 150 kDa
Exposure time: 1 minute 2 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721160) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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