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CD133 Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# ER31008

CD133 Rabbit Polyclonal Antibody

Application

  • WB

  • IHC-P

  • FC

Reactivity

  • Human

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

CD133 Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Recombinant protein within human CD133 aa 179-389.

Species Reactivity

Human

Validated Applications

WB, IHC-P, FC

Target Molecular Weight

Predicted band size: 97 kDa

Positive Control

Caco-2 cell lysate, HT-29 cell lysate, human breast tissue.

Conjugation

unconjugated

RRID

AB_3069558

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:1,000-1:5,000

  • IHC-P

  • 1:500-1:1,000

  • FC

  • 1:50-1:100

Target

Function

This gene encodes a pentaspan transmembrane glycoprotein. The protein localizes to membrane protrusions and is often expressed on adult stem cells, where it is thought to function in maintaining stem cell properties by suppressing differentiation. Mutations in this gene have been shown to result in retinitis pigmentosa and Stargardt disease. Expression of this gene is also associated with several types of cancer. This gene is expressed from at least five alternative promoters that are expressed in a tissue-dependent manner. Multiple transcript variants encoding different isoforms have been found for this gene.

Background References

1. Yin AH, Miraglia S, Zanjani ED,et al. CD133, a novel marker for human hematopoietic stem and progenitor cells. Blood,1997; 90:5002-5012

2. Wuchter C,Ratei R,Spahn G,et al. Impact of CD133 (CD133) and CD90 expression analysis for acute leukemia immunophenotyping. Haematologica, 2001; 86:154-161

3. Kuci S,Schumn M,Schlegel PG,et al. Phenotypic and functional characterization of mobilized peripheral blood CD133+CD34+ hematopoietic stem cell. Blood,1999; 94(Suppl 1):559a.

Sequence Similarity

Belongs to the prominin family.

Tissue Specificity

Isoform 1 is selectively expressed on CD34 hematopoietic stem and progenitor cells in adult and fetal bone marrow, fetal liver, cord blood and adult peripheral blood. Isoform 1 is not detected on other blood cells. Isoform 1 is also expressed in a number of non-lymphoid tissues including retina, pancreas, placenta, kidney, liver, lung, brain and heart. Found in saliva within small membrane particles. Isoform 2 is predominantly expressed in fetal liver, skeletal muscle, kidney, and heart as well as adult pancreas, kidney, liver, lung, and placenta. Isoform 2 is highly expressed in fetal liver, low in bone marrow, and barely detectable in peripheral blood. Isoform 2 is expressed on hematopoietic stem cells and in epidermal basal cells (at protein level). Expressed in adult retina by rod and cone photoreceptor cells (at protein level).

Post-translational Modification

Isoform 1 and isoform 2 are glycosylated.; Acetylation at Lys-225, Lys-257 and Lys-264 by NAT8 and NAT8B may control PROM1 protein expression and its function in cell apoptosis.

Subcellular Location

Cell membrane.

UNIPROT

SwissProt: O43490 Human

Synonyms

AC133 antibody

Antigen AC133 antibody

CD133 antibody

CORD12 antibody

Hematopoietic stem cell antigen antibody

hProminin antibody

MCDR2 antibody

MSTP061 antibody

OTTHUMP00000217744 antibody

OTTHUMP00000217745 antibody

Expand

Images

  • Western blot analysis of CD133 on different lysates with Rabbit anti-CD133 antibody (<a href="/products/ER31008" style="font-weight: bold;text-decoration: underline;">ER31008</a>) at 1/5,000 dilution.<br /><br />Lane 1: Caco-2 (Human colorectal adenocarcinoma cell) cell lysate<br />Lane 2: HT-29 (Human colorectal adenocarcinoma cell) cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br />Exposure time: 10 seconds; ECL: K1801<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/ER31008" style="font-weight: bold;text-decoration: underline;">ER31008</a>, 1/5,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 97.2 kDa<br />Observed band size: 120 kDa

    Western blot analysis of CD133 on different lysates with Rabbit anti-CD133 antibody (ER31008) at 1/5,000 dilution.

    Lane 1: Caco-2 (Human colorectal adenocarcinoma cell) cell lysate
    Lane 2: HT-29 (Human colorectal adenocarcinoma cell) cell lysate

    Lysates/proteins at 20 µg/Lane.
    Exposure time: 10 seconds; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: ER31008, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 97.2 kDa
    Observed band size: 120 kDa

  • Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-CD133 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ER31008" style="font-weight: bold;text-decoration: underline;">ER31008</a>, 1/800) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-CD133 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER31008, 1/800) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

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