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CD134 Mouse Monoclonal Antibody [2H1]

Usd: 350

Specification

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Catalog# EM1701-24

CD134 Mouse Monoclonal Antibody [2H1]

Application

  • WB

  • IHC-P

  • IF-Cell

  • FC

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

CD134 Mouse Monoclonal Antibody [2H1]

Antibody Type

Mouse Monoclonal Antibody

Immunogen

Recombinant fragment within Human CD134/ OX40L receptor aa 59-205.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P, IF-Cell, FC

Molecular Weight

Predicted band size: 29 kDa

Positive Control

A549 cells, Hela cells, HepG2 cells, rat lung tissue, rat spleen tissue, human tonsil tissue, human spleen tissue, mouse spleen tissue, HL-60 cells, mouse lung tissue lysates.

Conjugation

unconjugated

Clone Number

2H1

RRID

AB_3068752

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG1

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500

  • IF-Cell

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

Target

Function

Ox40 (also designated CD134 and Ox40R), is a member of the tumor necrosis factor receptor (TNFR) family. Ox40 is involved in coordinating CD4 T cell selection, migration and cytokine differentiation in T helper (Th)1 and Th2 cells. Ox40 is also involved in the stimulation of T cells, T cell-dependent humoral response and generation of optimal CD4+ T cell responses in vivo and in vitro. Ox40 is expressed on activated CD4+ T lymphocytes and its ligand, Ox40L, is found preferentially on activated B cells. Engagement of Ox40 with its ligand, Ox40L, delivers a strong costimulatory signal to effector T cells. Members of the TNFR superfamily are critically involved in the regulation of infections, inflammation, autoimmune diseases and tissue homeostasis.

Background References

1. Liu T et al. Immunomodulatory effects of OX40Ig gene-modified adipose tissue-derived mesenchymal stem cells on rat kidney transplantation. Int J Mol Med 39:144-152 (2017).

2. Wang X et al. Effect of cyclosporin A intervention on the immunological mechanisms of coronary heart disease and restenosis. Exp Ther Med 12:3242-3248 (2016).

Subcellular Location

Membrane.

UNIPROT

SwissProt: P43489 Human

SwissProt: P47741 Mouse

SwissProt: P15725 Rat

Synonyms

ACT 35 antibody

ACT35 antibody

ACT35 antigen antibody

ATC35 antigen antibody

CD 134 antibody

CD134 antibody

CD134 antigen antibody

IMD16 antibody

Lymphoid activation antigene ACT35 antibody

OX 40 antibody

Expand

Images

  • Western blot analysis of CD134 on mouse lung tissue lysates with Mouse anti-CD134 antibody (<a href="/products/EM1701-24" style="font-weight: bold;text-decoration: underline;">EM1701-24</a>) at 1/500 dilution.<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 29 kDa<br />Observed band size: 29 kDa<br /><br />Exposure time: 2 minutes;<br /><br />12% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/EM1701-24" style="font-weight: bold;text-decoration: underline;">EM1701-24</a>) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1:100,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of CD134 on mouse lung tissue lysates with Mouse anti-CD134 antibody (EM1701-24) at 1/500 dilution.

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 29 kDa
    Observed band size: 29 kDa

    Exposure time: 2 minutes;

    12% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-24) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.

  • ICC staining CD134 (green) in A549 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining CD134 (green) in A549 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • ICC staining CD134 (green) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining CD134 (green) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • ICC staining CD134 (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining CD134 (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Immunohistochemical analysis of paraffin-embedded rat lung tissue using anti-CD134 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded rat lung tissue using anti-CD134 antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded rat spleen tissue using anti-CD134 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue using anti-CD134 antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD134 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD134 antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD134 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD134 antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-CD134 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-CD134 antibody. Counter stained with hematoxylin.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

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